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AB319548

Alexa Fluor® 488 Anti-NXF1 antibody [EPR8009]

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Rabbit Recombinant Monoclonal NXF1 antibody - conjugated to Alexa Fluor® 488.

View Alternative Names

TAP, NXF1, Nuclear RNA export factor 1, Tip-associated protein, Tip-associating protein, mRNA export factor TAP

  • 660 APC

    APC Anti-NXF1 antibody [EPR8009]

  • 578 PE

    PE Anti-NXF1 antibody [EPR8009]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-NXF1 antibody [EPR8009]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-NXF1 antibody [EPR8009]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-NXF1 antibody [EPR8009]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8009

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NXF1 also known as TAP functions as an mRNA nuclear export factor. It plays a critical role in transporting mRNA from the nucleus to the cytoplasm. NXF1 protein mass is approximately 70 kDa. Researchers primarily observe its expression in various human tissues with higher expression in the liver and brain. The NXF1 protein interacts with nuclear pore complexes to facilitate mRNA transport making it important in gene expression regulation.
Biological function summary

This mRNA transport factor ensures correct mRNA localization and effective translation into proteins. NXF1 acts part of a multiprotein complex where it interacts with NXT1 to guide mature mRNA to the nuclear pore for export. This action is central for maintaining cellular protein homeostasis and enables proper cellular responses to different stimuli. Disruptions in NXF1 function can cause interruptions in the mRNA export process impacting protein synthesis.

Pathways

NXF1 takes part in essential cellular processes related to nucleocytoplasmic transport. It operates within the mRNA export pathway ensuring mRNAs navigate from the nucleus to the cytoplasm. NXF1 closely works with other export factors such as NUP98 in this pathway. Additionally it associates with the spliceosome helping to coordinate the export of correctly spliced mRNA which is important for efficient gene expression and cellular function.

NXF1 alterations have connections to certain types of cancer and neurological disorders. For instance malfunction in its mRNA export role can contribute to tumorigenesis as seen with breast cancer by misregulating gene expression. In neurological diseases like amyotrophic lateral sclerosis (ALS) defects in mRNA transport can impair neuronal function. Genetic disruptions impacting NXF1 dynamics often involve proteins like FUS which also participate in RNA metabolism and are linked to similar disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed : 10924507). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex (PubMed : 18364396, PubMed : 19165146, PubMed : 9660949). ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export (PubMed : 18364396, PubMed : 19165146, PubMed : 9660949). Also involved in nuclear export of m6A-containing mRNAs : interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).
See full target information NXF1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com