AB208907

Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Oct4 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980] (AB208907)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980] (AB208907)

ab208907 staining Oct4 in mouse Embryonic Stem cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208907 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in mouse Embryonic Stem cells fixed with 4% formaldehyde (10 min).

578 PE

PE Anti-Oct4 antibody [EPR17980]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Oct4 antibody [EPR17980]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Oct4 antibody [EPR17980]
Unconjugated

Anti-Oct4 antibody [EPR17980]
Carrier free

Anti-Oct4 antibody [EPR17980] - BSA and Azide free (Capture)
Carrier free

Anti-Oct4 antibody [EPR17980] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17980

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse, Human, Mouse

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in mouse Embryonic Stem cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.

Biological function summary

Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.

Pathways

Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.

Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

See full target information POU5F1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:8392313 PubMed35615580

2022

Extracellular Vesicle-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbing Zhu,Zhaoyang Chen,Xiaoping Peng,Zeqi Zheng,Aiping Le,Junjie Guo,Leilei Ma,Hongtao Shi,Kang Yao,Shuning Zhang,Junbo Ge,Zhenzhong Zheng,Qian Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980] (AB208907)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise