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Anti-Olig2 [EPR2673] Alexa Fluor® 488 conjugated antibody (ab225099) rabbit monoclonal antibody that is used to detect Olig2 in IHC-Fr. Suitable for Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (AB225099), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (AB225099), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (AB225099), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-Fr
Human
Predicted
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Rat
Dilution info
1/250
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

5 products for Alternative Version

Target data

Function

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Alternative names

BHLHB1, BHLHE19, PRKCBP2, RACK17, OLIG2, Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19

Recommended products

Anti-Olig2 [EPR2673] Alexa Fluor® 488 conjugated antibody (ab225099) rabbit monoclonal antibody that is used to detect Olig2 in IHC-Fr. Suitable for Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2673
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

What is this antibody validated in?


Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr), in Mouse, Rat samples.

Recommended positive controls


IHC-Fr: Normal rat brain tissue sections, mouse cerebrum (fresh frozen).

Related products


Antibody clone EPR2673 is also available pre-conjugated to a variety of labels for your convenience – Anti-Olig2 Alexa Fluor® 488 [EPR2673] (ab225099).

Other related products


We have a range of other formats of antibody clone [EPR2673] also available for your convenience:
Anti-Olig2 antibody [EPR2673] ab109186, Carrier free - Anti-Olig2 antibody [EPR2673] - BSA and Azide free ab220796, Alexa Fluor® 488 - ab225099, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] ab225100, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] ab300729



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Olig2 also known as Oligo2 or OLIG2 is a protein with a molecular mass of around 32 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which play roles in neurodevelopment. Olig2 is expressed mainly in the central nervous system particularly in oligodendrocyte progenitor cells and motor neuron progenitors. As an important marker Olig2 staining is often used in studies involving these cell types to understand differentiation processes.

Biological function summary

Olig2 regulates gene expression important for the proper development of the central nervous system. It functions both independently and as a part of transcriptional complexes to influence the fate of neural progenitors. In particular its presence decides whether these progenitors will differentiate into neurons astrocytes or oligodendrocytes. Among the proteins it interacts with are factors that modulate neurogenesis and myelination highlighting its importance in maintaining normal brain homeostasis.

Pathways

Olig2 plays an important role in the oligodendrocyte differentiation and motor neuron generation pathways. It works closely with proteins such as SOX10 and NKX2.2 to regulate oligodendrocyte maturation. In the motor neuron pathway Olig2 interacts with proteins like NKX6.1 and LHX3 coordinating the development of motor neurons. These pathways demonstrate Olig2's function as a critical transcription factor influencing neural development and plasticity.

Associated diseases and disorders

Abnormalities in Olig2 expression or function relate to conditions such as gliomas and Multiple Sclerosis (MS). Elevated levels of Olig2 are often seen in gliomas where it might cooperate with proteins like IDH1 to drive tumor growth. In MS the impairment of oligodendrocyte function can link back to disruptions in normal Olig2 activity or expression. These associations make Olig2 a significant target for potential therapeutic interventions in neurological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com