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AB202655

Alexa Fluor® 488 Anti-p150 CAF1/CAF antibody [EPR5576(2)]

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Rabbit Recombinant Monoclonal p150 CAF1/CAF antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CAF, CAF1P150, CHAF1A, Chromatin assembly factor 1 subunit A, CAF-1 subunit A, Chromatin assembly factor I p150 subunit, CAF-I 150 kDa subunit, CAF-I p150, hp150

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p150 CAF1/CAF antibody [EPR5576(2)] (AB202655)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p150 CAF1/CAF antibody [EPR5576(2)] (AB202655)

ab202655 staining p150 CAF1/CAF in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202655 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-p150 CAF1/CAF antibody [EPR5576(2)] (AB202655)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-p150 CAF1/CAF antibody [EPR5576(2)] (AB202655)

Overlay histogram showing HeLa cells stained with ab202655 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab202655, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Unconjugated

    Anti-p150 CAF1/CAF antibody [EPR5576(2)]

  • Carrier free

    Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5576(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p150 CAF1 also known as CAF protein and CAF1 protein is part of the chromatin assembly factor 1 complex. It weighs approximately 150 kDa. This protein primarily expresses in the nucleus of eukaryotic cells where it plays an important role in DNA replication and repair. The p150 protein acts as a structural component that is essential for proper chromatin assembly during the S phase of the cell cycle.
Biological function summary

This target functions as part of the CAF-1 complex which includes two additional subunits—p60 and p48. The complex aids in the assembly of newly synthesized histones onto replicating DNA facilitating nucleosome formation. It affects chromatin dynamics influencing transcriptional regulation by providing nucleosome assembly binding sites along DNA. This process maintains genomic integrity during cell division and influences gene expression in response to various cell signals.

Pathways

The p150 protein interacts within pathways involved in DNA replication and repair. It plays a major role in the nucleosome assembly pathway closely interacting with histone proteins H3 and H4. Through these interactions it ensures the stability of replication forks during DNA synthesis. Besides it participates in the regulation of the cell cycle and influences the chromatin assembly pathway important for accurate DNA replication and cell division.

Several conditions show connection with the p150 protein's function in DNA replication. Alterations or defects in its expression link to cancer where chromatin misassembly leads to genomic instability a hallmark of tumorigenesis. Additionally the involvement of p150 in neurodegenerative disorders sheds light on how disrupted chromatin assembly can affect neural cells. Its interaction with proteins like retinoblastoma (Rb) points to the potential dysregulation in cell cycle control further influencing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
See full target information CHAF1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB232030

Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free

primary-antibodies

p150-caf1-caf-antibody-epr55762-bsa-and-azide-free-ab232030

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com