Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] (AB309607)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling P2Y12 with ab309607 at 1/50 (10.0 ug/ml). Positive staining on rat cerebrum. The section was incubated with ab309607 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] (AB309607)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling P2Y12 with ab309607 at 1/50 (10.0 ug/ml). Positive staining on mouse cerebrum. The section was incubated with ab309607 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] (AB309607)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling P2Y12 with ab309607 at 1/50 (10.0 ug/ml) . Negative control : no staining on mouse liver. The section was incubated with ab309607 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] (AB309607)

Image A : composite multiplex immunohistochemistry staining using conjugates panel composed of anti-P2Y12 (Alexa Fluor^® 488) (ab309607, 1/50 dilution, 10 µg/mL) (green), anti-GFAP (Alexa Fluor^® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-NeuN (Alexa Fluor^® 647) (ab190565, 1/100 dilution, 5 µg/mL) (white) on Frozen sections of mouse cerebellum. Nuclear DNA was labelled with DAPI (blue).

Image B : Frozen section of mouse cerebellum stained with anti- P2Y12 (Alexa Fluor^® 488) (ab309607, 1/50 dilution, 10 µg/mL).

Image C : Frozen section of mouse cerebellum stained with anti-GFAP (Alexa Fluor^® 555) (ab201735, 1/100 dilution, 5 µg/mL).

Image D : Frozen section of mouse cerebellum stained with anti-NeuN (Alexa Fluor^® 647) (ab190565, 1/100 dilution, 5 µg/mL).

Immunohistochemistry staining was performed on a Leica Biosystems BOND^® RX instrument. The section was initially fixed using 10% formaldehyde in 1XPBS for 10 minutes. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium^®.

Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.