Rabbit Recombinant Monoclonal PACAP antibody - conjugated to Alexa Fluor® 488.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation. Acts as a hormone-regulated adipokine/pro-inflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.
MEDA7, PACAP, HSPC190, MZB1, Marginal zone B- and B1-cell-specific protein, Mesenteric estrogen-dependent adipose 7, Plasma cell-induced resident endoplasmic reticulum protein, Proapoptotic caspase adapter protein, MEDA-7, Plasma cell-induced resident ER protein, pERp1
Rabbit Recombinant Monoclonal PACAP antibody - conjugated to Alexa Fluor® 488.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
PACAP also known as Pituitary Adenylate Cyclase-Activating Polypeptide is a neuropeptide functioning as a neurotransmitter and neuromodulator. It belongs to the vasoactive intestinal peptide (VIP)/secretin/glucagon hormone family. PACAP consists of 38 amino acid residues and has a molecular mass of approximately 4.5 kDa. It is expressed in various regions including the central nervous system (CNS) endocrine cells and peripheral tissues. This broad expression pattern supports its diverse role in physiological and neurobiological processes.
PACAP exerts multiple effects by binding to three G protein-coupled receptors: PAC1 VPAC1 and VPAC2. It influences processes like neuroprotection neurodevelopment and neurotransmission. As part of the PACAP/VIP receptor complex it regulates cyclic AMP production and calcium signaling mediating its effects in both neural and non-neural tissues. It is particularly involved in paired functions with neurotransmitters and other signaling molecules indicating its key role in maintaining homeostasis.
The PACAP signaling mechanism integrates into the cAMP-dependent pathway and the MAPK/ERK pathway. These pathways are central to cell proliferation differentiation and survival. PACAP's interaction with MAPK/ERK pathway involves proteins such as Raf and MEK further modulating gene expression and synaptic plasticity. By this mechanism PACAP plays a vital role in modulating neurotransmission and neuroendocrine functions.
PACAP is linked to migraine and neurodegenerative diseases like Alzheimer's disease. Alterations in PACAP signaling can affect pain pathways and neuroinflammation potentially exacerbating such conditions. PACAP's interaction with proteins like CGRP (Calcitonin Gene-Related Peptide) is significant as both are implicated in the pathophysiology of migraines. Moreover in Alzheimer’s disease the dysregulation of PACAP and its receptors contributes to neuroinflammation and neurodegeneration.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com