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AB277270

Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11]

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(4 Publications)

Anti-pan Cytokeratin antibody [C-11] - Alexa Fluor® 488 conjugated (ab277270) is a mouse recombinant monoclonal antibody detecting pan Cytokeratin in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

2 Images
Immunocytochemistry - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)
  • ICC

Lab

Immunocytochemistry - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

ab277270 staining pan Cytokeratin cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab277270 at at 1µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 minutes).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative Jurkat cells stained with ab277270 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab277270) (1x 106 in 100μL at 0.2μg/mL (1/2500)) for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1κ Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Unconjugated

    Anti-pan Cytokeratin antibody [C-11]

  • 660 APC

    APC Anti-pan Cytokeratin antibody [C-11]

  • 578 PE

    PE Anti-pan Cytokeratin antibody [C-11]

  • Carrier free

    Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-pan Cytokeratin antibody [C-11]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-pan Cytokeratin antibody [C-11]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-pan Cytokeratin antibody [C-11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-11

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (ab277270) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone C-11 is also available pre-conjugated to a variety of labels for your convenience – Anti-pan Cytokeratin Alexa Fluor® 488 [C-11] (ab277270).

Other related products
We have a range of other formats of antibody clone [C-11] also available for your convenience: ab7753, PE - ab52460, APC - ab106166, Carrier free - ab264485, ab269366, Alexa Fluor® 594 - ab277234, Alexa Fluor® 488 - ab277270, Alexa Fluor® 555 - ab279324, Oligonucleotide - ab279360, Alexa Fluor® 647 - ab309978

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
See full target information KRT1

Additional targets

KRT7,KRT4,KRT19,Keratin, type I cytoskeletal 9,KRT17,KRT3,Keratin, type I cytoskeletal 14,KRT6C,KRT6B,KRT20,KRT18,Keratin, type II cytoskeletal 5,KRT8,KRT16,KRT10,KRT15,Keratin, type I cytoskeletal 13,KRT12,KRT6A,KRT2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biology open 12: PubMed37194999

2023

Bioengineering of a tumour-stroma 3D-tumouroid co-culture model of hypopharyngeal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Santu Saha,Rachel Howarth,Sweta Sharma-Saha,Charles Kelly

International journal of molecular sciences 24: PubMed37108635

2023

Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and -MSH for Skin Whitening.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Yang,Zhekun Wang,Yunwei Hu,Kaile Zong,Xingjiang Zhang,Hui Ke,Pan Wang,Yuyo Go,Xi Hui Felicia Chan,Jianxin Wu,Qing Huang

Science advances 8:eabo7792 PubMed36054348

2022

Genome-wide in vivo screen of circulating tumor cells identifies as a regulator of metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Xia,Yuan Ma,Kangfu Chen,Bill Duong,Sharif Ahmed,Randy Atwal,David Philpott,Troy Ketela,Jennifer Pantea,Sichun Lin,Stephane Angers,Shana O Kelley

Reproduction & fertility 3:30-38 PubMed35350653

2022

Altered endometrial oestrogen-responsiveness and recurrent reproductive failure.

Applications

Unspecified application

Species

Unspecified reactive species

Hannan Al-Lamee,Amy Ellison,Josephine Drury,Christopher J Hill,Andrew J Drakeley,Dharani K Hapangama,Nicola Tempest
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB52460

PE Anti-pan Cytokeratin antibody [C-11]

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pe-pan-cytokeratin-antibody-c-11-ab52460

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Alternative Version
Primary Antibodies

AB264485

Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free

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pan-cytokeratin-antibody-c-11-bsa-and-azide-free-ab264485

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Alternative Version
Primary Antibodies

AB309978

Alexa Fluor® 647 Anti-pan Cytokeratin antibody [C-11]

primary-antibodies

alexa-fluor-647-pan-cytokeratin-antibody-c-11-ab309978

0

(0 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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