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AB309720

Alexa Fluor® 488 Anti-PCBP2/hnRNP E2 antibody [EPR14858]

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Rabbit Recombinant Monoclonal PCBP2/hnRNP E2 antibody - conjugated to Alexa Fluor® 488.

View Alternative Names

Poly(rC)-binding protein 2, Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2, PCBP2

  • 578 PE

    PE Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • 660 APC

    APC Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • HRP

    HRP Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PCBP2/hnRNP E2 antibody [EPR14858]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PCBP2/hnRNP E2 antibody [EPR14858]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14858

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCBP2 also known as hnRNP E2 is a poly(C) binding protein with a molecular weight of approximately 38 kDa. It binds to single-stranded poly(C) sequences in RNA molecules. PCBP2 is widely expressed in various tissues where it modulates pre-mRNA splicing RNA stability and translation. It functions as part of ribonucleoprotein complexes and is known for its role in regulating mRNA transport and localization within the cell.
Biological function summary

A significant function of PCBP2 involves the maintenance of mRNA stability particularly for certain growth factor and cytokine mRNAs. PCBP2 is part of the ribonucleosome complex supporting various cellular processes by regulating the turnover of target mRNAs. Through these actions it helps control cellular proliferation and immune responses. PCBP2 collaborates with several other proteins influencing cellular adaptation to stress and changes in the environment.

Pathways

PCBP2 plays key roles in the Wnt signaling and Toll-like receptor pathways. In the Wnt signaling pathway its interaction with mRNA targets such as beta-catenin is significant for cellular communication processes. In the Toll-like receptor pathway it works alongside proteins like MyD88 modulating innate immune responses to pathogens. PCBP2's participation in these pathways highlights its versatility and influence on cellular and systemic communication networks.

Researchers have linked PCBP2 to cancer particularly due to its involvement with oncogene regulation. Disruptions in the expression of PCBP2 can affect cell cycle control contributing to tumorigenesis. PCBP2 also connects with amyotrophic lateral sclerosis (ALS) through its interaction with proteins like TDP-43 which are critical in neurodegenerative disease pathways. Understanding PCBP2's function and its connections to these conditions remains important for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed : 12414943, PubMed : 7607214). Major cellular poly(rC)-binding protein (PubMed : 12414943). Binds also poly(rU) (PubMed : 12414943). Acts as a negative regulator of antiviral signaling (PubMed : 19881509, PubMed : 35322803). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed : 19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed : 19881509). Negativeley regulates the cGAS-STING pathway via interaction with CGAS, preventing the formation of liquid-like droplets in which CGAS is activated (PubMed : 35322803). Together with PCBP1, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).. (Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed : 12414943, PubMed : 24371074). Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed : 12414943).
See full target information PCBP2
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