Mouse Monoclonal PCNA antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 7 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (AB201672), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pcna-antibody-pc10-ab201672--immunocytochemistry-immunofluorescence-img66299.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (AB201672)")
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (AB201672), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pcna-antibody-pc10-ab201672--flow-cytometry-intracellular-img6904.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (AB201672)")
Publications
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- Frontiers in immunology 13:8652732022Milk Fat Globule Membrane Attenuates Acute Colitis and Secondary Liver Injury by Improving the Mucus Barrier and Regulating the Gut Microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhua Wu,Xiaoyi Liu,Shimeng Huang,Tiantian Li,Xiangyu Zhang,Jiaman Pang,Junying Zhao,Lijun Chen,Bing Zhang,Junjun Wang,Dandan HanPubMed 35799795
- The FEBS journal 289:1625-16492021Cytosolic localization and in vitro assembly of human de novo thymidylate synthesis complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharon Spizzichino,Dalila Boi,Giovanna Boumis,Roberta Lucchi,Francesca Romana Liberati,Davide Capelli,Roberta Montanari,Giorgio Pochetti,Roberta Piacentini,Giacomo Parisi,Alessio Paone,Serena Rinaldo,Roberto Contestabile,Angela Tramonti,Alessandro Paiardini,Giorgio Giardina,Francesca CutruzzolàPubMed 34694685
- EMBO reports 21:e483892020Homeobox A4 suppresses vascular remodeling by repressing YAP/TEAD transcriptional activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Kimura,Takahiro Horie,Osamu Baba,Yuya Ide,Shuhei Tsuji,Randolph Ruiz Rodriguez,Toshimitsu Watanabe,Tomohiro Yamasaki,Chiharu Otani,Sijia Xu,Yui Miyasaka,Yasuhiro Nakashima,Takeshi Kimura,Koh OnoPubMed 32147946
- Cell reports 27:1794-1808.e52019Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Huang,Jing Zhang,Marina A Bellani,Durga Pokharel,Julia Gichimu,Ryan C James,Himabindu Gali,Chen Ling,Zhijiang Yan,Dongyi Xu,Junjie Chen,Amom Ruhikanta Meetei,Lei Li,Weidong Wang,Michael M SeidmanPubMed 31067464
- Nature communications 10:1192019Ultrafast data mining of molecular assemblies in multiplexed high-density super-resolution images.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYandong Yin et. al.PubMed 30631072
- International journal of oncology 53:1713-17202018Anaplastic transition within the cancer microenvironment in early-stage oral tongue squamous cell carcinoma is associated with local recurrence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKohei Okuyama et. al.PubMed 30085337
- Oncotarget 7:26979-912016Characterization of cytoplasmic cyclin D1 as a marker of invasiveness in cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoel P Fusté,Esmeralda Castelblanco,Isidre Felip,Maria Santacana,Rita Fernández-Hernández,Sònia Gatius,Neus Pedraza,Judit Pallarés,Tània Cemeli,Joan Valls,Marc Tarres,Francisco Ferrezuelo,Xavier Dolcet,Xavier Matias-Guiu,Eloi GaríPubMed 27105504
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Catshark | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dogfish | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Pigeon | Predicted | Predicted |
| Thornback ray | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pigeon, Pig, Drosophila melanogaster, Monkey, Zebrafish, Thornback ray, Dogfish, Catshark | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pigeon, Pig, Drosophila melanogaster, Monkey, Zebrafish, Thornback ray, Dogfish, Catshark | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
6 products for Alternative Product
Target data
Function
Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Recommended products
Mouse Monoclonal PCNA antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Clone number
PC10
- Purification technique
Affinity purification
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
- Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
- Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
- Associated diseases and disorders
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pcna-antibody-pc10-ab201672--immunocytochemistry-immunofluorescence-img66299.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672)
ab201672 staining PCNA in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201672 at 1/200 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pcna-antibody-pc10-ab201672--flow-cytometry-intracellular-img6904.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672)")
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672)
Overlay histogram showing HeLa cells stained with ab201672 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab201672, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a (monoclonal) Alexa Fluor® 488 (ab171464) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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