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Rabbit Recombinant Monoclonal PD-L1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line, Human samples. Cited in 7 publications.


Images

Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt
Human
Expected
Expected
Transfected cell line
Tested
Tested

Tested
Tested

Species

Transfected cell line

Dilution info

1/100

Notes

This product gave a positive signal in PDL1 transfected CHO cells fixed with 4% formaldehyde (10 min)

Expected
Expected

Species

Human

Dilution info

1/100

Notes

This product gave a positive signal in PDL1 transfected CHO cells fixed with 4% formaldehyde (10 min)

Tested
Tested

Species

Transfected cell line

Dilution info

1/50

Notes

-

Expected
Expected

Species

Human

Dilution info

1/50

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Version

15 products for Alternative Product

Target data

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PD-L1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line, Human samples. Cited in 7 publications.

Alternative names

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

28-8

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565).

Additional information on positive controls:

Cell Lines:
Positives: B-CPAP- high, ES-2- medium, HCC70 - low

For primary negative control, isotype control, RabMAb negative control antibody (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) is recommended.

For negative control sample, cell line COLO205 is recommended.

For PD-L1 protein, see Recombinant human PD-L1 protein (Active) ab167713.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

    Overlay histgram showing CHO (blue line) and PDL1 transfected CHO (red line) cells stained with ab209959.

    The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209959, 1/50 dilution) for 30 min at 4°C.

    Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

    ab209959 staining PDL1 in CHO-PDL1 cells. The lower panels demonstrate that ab209959 does not cross react with un-transfected CHO cells.

    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209959 at a 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com