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AB209959

Alexa Fluor® 488 Anti-PD-L1 antibody [28-8]

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(1 Review)

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(10 Publications)

Anti-PD-L1 antibody [28-8] - Alexa Fluor® 488 conjugated (ab209959) is a rabbit recombinant monoclonal antibody detecting PD-L1 in Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

2 Images
Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959)

Overlay histgram showing CHO (blue line) and PDL1 transfected CHO (red line) cells stained with ab209959.

The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209959, 1/50 dilution) for 30 min at 4°C.

Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959)

ab209959 staining PDL1 in CHO-PDL1 cells. The lower panels demonstrate that ab209959 does not cross react with un-transfected CHO cells.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209959 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 660 APC

    APC Anti-PD-L1 antibody [28-8]

  • 578 PE

    PE Anti-PD-L1 antibody [28-8]

  • 519 FITC

    FITC Anti-PD-L1 antibody [28-8]

  • HRP

    HRP Anti-PD-L1 antibody [28-8]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PD-L1 antibody [28-8]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PD-L1 antibody [28-8]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PD-L1 antibody [28-8]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain

  • Biotin

    Biotin Anti-PD-L1 antibody [28-8] (Detector)

  • Unconjugated

    Anti-PD-L1 antibody [28-8]

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector)

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free

  • Carrier free

    Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

28-8

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in PDL1 transfected CHO cells fixed with 4% formaldehyde (10 min)</p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" }, "Transfected cell line": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in PDL1 transfected CHO cells fixed with 4% formaldehyde (10 min)</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone 28-8 is also available pre-conjugated to a variety of labels for your convenience – Anti-PD-L1 Alexa Fluor® 488 [28-8] (ab209959).

Other related products
We have a range of other formats of antibody clone [28-8] also available for your convenience: ab205921, APC - ab206967, Carrier free - ab209889, Alexa Fluor® 488 - ab209959, Alexa Fluor® 647 - ab209960, HRP - ab209961, PE - ab209962, Alexa Fluor® 555 - ab213358, Alexa Fluor® 568 - ab213359, Alexa Fluor® 594 - ab213360, FITC - ab224027, Carrier free - ab228413, Carrier free - ab278010, Alexa Fluor® 647 - ab303724, Carrier free - ab307495, Biotin - ab322788

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Inflammation 48:975-986 PubMed39174864

2024

Optimal Timing of PD-1/PD-L1 Blockade Protects Organ Function During Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiang,Yuanyang Wang,Langtian Shi,Jinchao Qiu,Lebin Gan,Zhe Xu,Huacai Zhang,Jin Deng,Zhen Wang,Fang Xu,Ling Zeng

Journal of biochemical and molecular toxicology 38:e23682 PubMed38462752

2024

CLDN6 inhibited cellular biological function of nonsmall cell lung cancer cells through suppressing aerobic glycolysis via the RIP1/ASK1/JNK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Guo,Jianying Li,Yu Dong,Humei Gao,Peng Wang

Frontiers in oncology 13:1259314 PubMed38053658

2023

Novel combinatorial therapy of oncolytic adenovirus AdV5/3-D24-ICOSL-CD40L with anti PD-1 exhibits enhanced anti-cancer efficacy through promotion of intratumoral T-cell infiltration and modulation of tumour microenvironment in mesothelioma mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Mariangela Garofalo,Magdalena Wieczorek,Ines Anders,Monika Staniszewska,Michal Lazniewski,Marta Prygiel,Aleksandra Anna Zasada,Teresa Szczepińska,Dariusz Plewczynski,Stefano Salmaso,Paolo Caliceti,Vincenzo Cerullo,Ramon Alemany,Beate Rinner,Katarzyna Pancer,Lukasz Kuryk

Journal of experimental & clinical cancer research : CR 42:304 PubMed37974213

2023

Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Anand Singh,Nathanael Pruett,Shivani Dixit,Sudheer K Gara,Haitao Wang,Roma Pahwa,David S Schrump,Chuong D Hoang

Biomolecules 13: PubMed37189383

2023

Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique Heymann

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng

Cancer discovery 12:1482-1499 PubMed35254416

2022

Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Holly K Koblish,Liangxing Wu,Liang-Chuan S Wang,Phillip C C Liu,Richard Wynn,Jonathan Rios-Doria,Susan Spitz,Hao Liu,Alla Volgina,Nina Zolotarjova,Kanishk Kapilashrami,Elham Behshad,Maryanne Covington,Yan-Ou Yang,Jingwei Li,Sharon Diamond,Maxim Soloviev,Kevin O'Hayer,Stephen Rubin,Chrysi Kanellopoulou,Gengjie Yang,Mark Rupar,Darlise DiMatteo,Luping Lin,Christina Stevens,Yue Zhang,Pramod Thekkat,Ryan Geschwindt,Cindy Marando,Swamy Yeleswaram,Jeff Jackson,Peggy Scherle,Reid Huber,Wenqing Yao,Gregory Hollis

Bioengineered 12:11329-11341 PubMed34872456

2021

Paeoniflorin ameliorates chronic stress-induced depression-like behavior in mice model by affecting ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Tang,Min Chen,Qiang Li

British journal of cancer 124:484-493 PubMed33024266

2020

Lysine demethylase 2A expression in cancer-associated fibroblasts promotes breast tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Yi Chen,Chien-Feng Li,You-Syuan Lai,Wen-Chun Hung

Biomaterials 192:128-139 PubMed30448697

2018

Autophagy inhibitor enhance ZnPc/BSA nanoparticle induced photodynamic therapy by suppressing PD-L1 expression in osteosarcoma immunotherapy.

Applications

Flow Cyt

Species

Unspecified reactive species

Wei Yu,Yitian Wang,Jian Zhu,Libin Jin,Bing Liu,Kaishun Xia,Junjie Wang,Jianqing Gao,Chengzhen Liang,Huimin Tao
View all publications
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Product promise

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