Rabbit Recombinant Monoclonal PD-L1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line, Human samples. Cited in 7 publications.
Images
![Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pd-l1-antibody-28-8-ab209959--flow-cytometry-img78829.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959)")
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pd-l1-antibody-28-8-ab209959--immunocytochemistry-immunofluorescence-img63702.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (AB209959)")
Publications
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- Frontiers in oncology 13:12593142023Novel combinatorial therapy of oncolytic adenovirus AdV5/3-D24-ICOSL-CD40L with anti PD-1 exhibits enhanced anti-cancer efficacy through promotion of intratumoral T-cell infiltration and modulation of tumour microenvironment in mesothelioma mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariangela Garofalo,Magdalena Wieczorek,Ines Anders,Monika Staniszewska,Michal Lazniewski,Marta Prygiel,Aleksandra Anna Zasada,Teresa Szczepińska,Dariusz Plewczynski,Stefano Salmaso,Paolo Caliceti,Vincenzo Cerullo,Ramon Alemany,Beate Rinner,Katarzyna Pancer,Lukasz KurykPubMed 38053658
- Journal of experimental & clinical cancer research : CR 42:3042023Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnand Singh,Nathanael Pruett,Shivani Dixit,Sudheer K Gara,Haitao Wang,Roma Pahwa,David S Schrump,Chuong D HoangPubMed 37974213
- Biomolecules 13:2023Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPerrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique HeymannPubMed 37189383
- Cancer discovery 12:1482-14992022Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHolly K Koblish,Liangxing Wu,Liang-Chuan S Wang,Phillip C C Liu,Richard Wynn,Jonathan Rios-Doria,Susan Spitz,Hao Liu,Alla Volgina,Nina Zolotarjova,Kanishk Kapilashrami,Elham Behshad,Maryanne Covington,Yan-Ou Yang,Jingwei Li,Sharon Diamond,Maxim Soloviev,Kevin O'Hayer,Stephen Rubin,Chrysi Kanellopoulou,Gengjie Yang,Mark Rupar,Darlise DiMatteo,Luping Lin,Christina Stevens,Yue Zhang,Pramod Thekkat,Ryan Geschwindt,Cindy Marando,Swamy Yeleswaram,Jeff Jackson,Peggy Scherle,Reid Huber,Wenqing Yao,Gregory HollisPubMed 35254416
- Bioengineered 12:11329-113412021Paeoniflorin ameliorates chronic stress-induced depression-like behavior in mice model by affecting ERK1/2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiling Tang,Min Chen,Qiang LiPubMed 34872456
- British journal of cancer 124:484-4932020Lysine demethylase 2A expression in cancer-associated fibroblasts promotes breast tumour growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Yi Chen,Chien-Feng Li,You-Syuan Lai,Wen-Chun HungPubMed 33024266
- Biomaterials 192:128-1392018Autophagy inhibitor enhance ZnPc/BSA nanoparticle induced photodynamic therapy by suppressing PD-L1 expression in osteosarcoma immunotherapy.Applications:Flow CytReactive species:Unspecified reactive speciesWei Yu,Yitian Wang,Jian Zhu,Libin Jin,Bing Liu,Kaishun Xia,Junjie Wang,Jianqing Gao,Chengzhen Liang,Huimin TaoPubMed 30448697
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | |
|---|---|---|
| Human | Expected | Expected |
| Transfected cell line | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/100 | Notes This product gave a positive signal in PDL1 transfected CHO cells fixed with 4% formaldehyde (10 min) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in PDL1 transfected CHO cells fixed with 4% formaldehyde (10 min) |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Version
15 products for Alternative Product
Target data
Function
Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
Alternative names
Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, PDL1, B7H1, CD274, PDCD1LG1, PDCD1L1
Recommended products
Rabbit Recombinant Monoclonal PD-L1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line, Human samples. Cited in 7 publications.
Alternative names
Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, PDL1, B7H1, CD274, PDCD1LG1, PDCD1L1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
28-8
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565).
Additional information on positive controls:
Cell Lines:
Positives: B-CPAP- high, ES-2- medium, HCC70 - low
For primary negative control, isotype control, RabMAb negative control antibody (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) is recommended.
For negative control sample, cell line COLO205 is recommended.
For PD-L1 protein, see Recombinant human PD-L1 protein (Active) ab167713.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pd-l1-antibody-28-8-ab209959--flow-cytometry-img78829.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)")
Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
Overlay histgram showing CHO (blue line) and PDL1 transfected CHO (red line) cells stained with ab209959.
The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209959, 1/50 dilution) for 30 min at 4°C.
Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-pd-l1-antibody-28-8-ab209959--immunocytochemistry-immunofluorescence-img63702.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
ab209959 staining PDL1 in CHO-PDL1 cells. The lower panels demonstrate that ab209959 does not cross react with un-transfected CHO cells.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209959 at a 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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