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Rabbit Monoclonal PD1 antibody - conjugated to Alexa Fluor® 488.

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Images

Flow Cytometry - Alexa Fluor® 488 Anti-PD1 antibody [EPR26302-8] (AB323899), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Target data

Function

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11209085, PubMed:11698646, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:18287011, PubMed:18641123, PubMed:22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

Alternative names

CD279, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1

Rabbit Monoclonal PD1 antibody - conjugated to Alexa Fluor® 488.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26302-8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Alexa Fluor® 488 Anti-PD1 antibody [EPR26302-8] (ab323899), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-PD1 antibody [EPR26302-8] (ab323899)

    PD1 Flow Cytometry staining using rabbit Anti-PD1 antibody

    Flow cytometric analysis of CTLL-2 (mouse T lymphocyte, Left) / EL4 (mouse lymphoma T lymphocyte, Right) cells labelling PD1 with ab323899 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

    Negative control: CTLL-2 (PMID: 1396582).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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