AB322239

Alexa Fluor® 488 Anti-Perilipin-1 antibody [EPR3753(2)]

Recombinant
RabMAb
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Perilipin-1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

PERI, PLIN, PLIN1, Perilipin-1, Lipid droplet-associated protein

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Perilipin-1 antibody [EPR3753(2)] (AB322239)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Perilipin-1 antibody [EPR3753(2)] (AB322239)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Perilipin-1 with ab322239 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Perilipin-1 antibody [EPR3753(2)] (AB322239)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Perilipin-1 with ab322239 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in differentiated 3T3-L1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3753(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

You may be interested in:

AB185433

Lipolysis Assay Kit (Colorimetric)

1 Reviews

View product

Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perilipin-1 also known as PLIN1 is a protein with a mass of approximately 57 kDa. It plays an important role in lipid metabolism within adipose tissue. This protein is mainly found in adipocytes where it coats lipid droplets. By controlling the access of lipases to the lipid core Perilipin-1 regulates lipolysis. It prevents unnecessary breakdown of lipids by acting as a barrier and ensuring the protection and storage of energy reserves. This targeted regulation is important for maintaining energy homeostasis in the body.

Biological function summary

Perilipin-1 influences cellular energy balance and fat storage. It is an important member of the PAT family of proteins which includes perilipin A. Perilipin-1 does not typically form part of a larger protein complex; however it works closely with other proteins on the surface of lipid droplets. Its presence on the droplet surface is essential for controlling lipid mobilization particularly during fasting or energy-deprived states when organisms require a mechanism to access stored energy efficiently.

Pathways

The activity of Perilipin-1 is important in the lipolytic pathway. This pathway is involved in hydrolyzing stored triglycerides into free fatty acids and glycerol an essential process in energy metabolism. Perilipin-1 acts in tandem with hormone-sensitive lipase (HSL) to regulate this pathway. Upon activation by adrenaline or other stimuli perilipin-1 undergoes phosphorylation which in turn facilitates HSL translocation to the lipid droplet and enhances lipolysis. Besides its direct role Perilipin-1 also interacts with adipose triglyceride lipase (ATGL) further impacting lipid breakdown processes.

The dysfunction of Perilipin-1 can lead to metabolic diseases like obesity and type 2 diabetes. Abnormal regulation or mutations in the Perilipin-1 gene have been observed in these conditions affecting normal lipid metabolism. Disordered Perilipin-1 function can lead to excessive lipid storage impaired energy mobilization and increased insulin resistance. Additionally Perilipin-1 interacts indirectly with other proteins such as perilipin-2 which can also influence the development of these metabolic disorders. Understanding these interactions helps in exploring therapeutic strategies aimed at targeting lipid metabolism and related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.

See full target information PLIN1

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

style

right-column

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com