JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202671

Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal PI 3 Kinase catalytic subunit alpha/PIK3CA antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, p110alpha, PIK3CA

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (AB202671)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (AB202671)

ab202671 staining PI 3 Kinase catalytic subunit alpha/PIK3CA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202671 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Unconjugated

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]

  • Carrier free

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP383Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 kinase catalytic subunit alpha also known as PIK3CA or PI3K-alpha is a protein kinase with significant roles in cellular signaling. This protein has a mass of approximately 124 kDa and is part of the PI3 kinase family. PIK3CA is primarily expressed in various tissues including the liver breast and pancreas where it plays an important role in the regulation of cell growth and survival. The structure of PI3 kinase involves a complex of p110 catalytic subunits and p85 regulatory subunits which facilitate its enzymatic functions.
Biological function summary

The PIK3CA protein phosphorylates the inositol ring of phosphoinositides generating phosphatidylinositol (345)-trisphosphate (PIP3) a lipid second messenger involved in numerous signaling pathways. PIK3CA often acts within a complex interacting with regulatory subunits such as p85 to control its activity. The complex formation enhances its stability and localization to the cell membrane where it can exert its enzymatic actions effectively. In its biological capacity PIK3CA plays a role in cellular proliferation survival and metabolism.

Pathways

The PI3K/Akt signaling pathway involves PIK3CA as an important mediator. This pathway is integral to numerous cellular processes including metabolism growth and survival. PIK3CA links closely with Akt a downstream protein kinase that propagates the signal initiated by PIP3 generation. The mTOR pathway is another critical route involving PIK3CA where it regulates translation and cellular growth. These pathways emphasize the importance of PIK3CA in maintaining cellular homeostasis and responding to external growth signals.

Mutations and aberrations in PIK3CA associate with several cancers especially breast and colorectal cancers. The altered activity of PIK3CA can cause uncontrolled cell proliferation due to constant activation of the PI3K/Akt signaling pathway. This disrupts normal cellular apoptosis and fosters tumor growth. Additionally PIK3CA mutations are linked to certain genetic disorders such as PIK3CA-related overgrowth spectrum (PROS) where overactivation alters normal tissue growth and development. The interaction of PIK3CA with proteins like PTEN a tumor suppressor involved in cancer pathways also highlights its significance in oncogenic processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396, PubMed : 23936502, PubMed : 28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396, PubMed : 28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed : 23936502, PubMed : 28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
See full target information PIK3CA
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 290:4562-4576 PubMed37255287

2023

PTPN22 activates the PI3K pathway via 14-3-3τ in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Bai,Tao Wang,Xiaonan Zhang,Xinlei Ba,Na Zhang,Yanjiao Zhao,Xipeng Wang,Yang Yu,Bing Wang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB135384

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

  • 0
  • 0
Primary Antibodies

AB135371

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139], prediluted

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139], prediluted (AB135371)

  • 0
  • 0
Primary Antibodies

AB183957

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (AB183957)

  • 0
  • 0
Primary Antibodies

AB223532

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free (AB223532)

  • 0
  • 0
Primary Antibodies

AB243914

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (AB243914)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2
Primary Antibodies

AB38449

Anti-AKT (phospho T308) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT (phospho T308) antibody (AB38449)

  • 3
  • 5
Primary Antibodies

AB180967

Anti-PI 3 Kinase p85 beta antibody [EPR18416]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (AB180967)

  • 0
  • 0
Primary Antibodies

AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

20ul selling size|Recombinant

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com