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Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB225371), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB225371), expandable thumbnail

Publications

  • The FEBS journal 290:4562-45762023
    PTPN22 activates the PI3K pathway via 14-3-3τ in T cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Bin Bai,Tao Wang,Xiaonan Zhang,Xinlei Ba,Na Zhang,Yanjiao Zhao,Xipeng Wang,Yang Yu,Bing Wang
    PubMed 37255287

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

This product gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

12 products for Alternative Product

    4 products for Alternative Version

      Target data

      Function

      Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

      Alternative names

      Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1

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      Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

      Alternative names

      Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1

      Key facts

      Isotype

      IgG

      Conjugation

      Alexa Fluor® 488

      Excitation/Emission

      Ex: 495nm, Em: 519nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR18702

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Supplementary info

      Activity summary

      PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.

      Biological function summary

      P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.

      Pathways

      PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.

      Associated diseases and disorders

      Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      2 product images

      • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225371), expandable thumbnail

        Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225371)

        Overlay histogram showing Jurkat cells stained with ab225371 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225371, 1/500 dilution) for 30 min at 22°C

        Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225371), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225371)

        ab225371 staining PI 3 Kinase p85 alpha (phospho Y607) in Jurkat cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225371 at 1/1000 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com