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Rabbit Recombinant Monoclonal PPP1R1A antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PPP1R1A antibody [EPR28192-90] (AB323837), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PPP1R1A antibody [EPR28192-90] (AB323837), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.

Alternative names

Protein phosphatase 1 regulatory subunit 1A, Protein phosphatase inhibitor 1, I-1, IPP-1

Rabbit Recombinant Monoclonal PPP1R1A antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28192-90
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein phosphatase 1 regulatory inhibitor subunit 1A also known as PPP1R1A or inhibitor-1 (I-1) plays a significant role in cellular regulation as a phosphatase inhibitor. Mechanically it regulates the activity of protein phosphatase 1 (PP1) an essential enzyme in various cellular processes. Its molecular weight is approximately 19 kDa. PPP1R1A is expressed in many tissues showing notable levels in cardiac and skeletal muscle where it helps modulate responses to various stimuli.

Biological function summary

PPP1R1A modulates signaling pathways by influencing the dephosphorylation of substrates which is a critical step in signal transduction. It is not typically part of a complex but interacts closely with protein phosphatase 1. This interaction allows for the effective regulation of phosphorylation states within cells impacting cell cycle control muscle contraction glycogen metabolism and more. Its regulatory capabilities are important for maintaining cellular homeostasis.

Pathways

The regulatory activities of PPP1R1A significantly impact the cAMP signaling pathway and the regulation of glycogen metabolism. Within the cAMP pathway PPP1R1A regulates the activity of PP1 influencing processes like muscle contraction and gene expression. It interacts with proteins such as PKA which phosphorylates PPP1R1A activating its inhibitory function. This regulation affects downstream cellular activities by modulating phosphorylation levels of key substrates within these pathways.

Associated diseases and disorders

Alterations in PPP1R1A expression or function have implications in cardiac diseases and diabetes. Increased or decreased activity of PPP1R1A can disrupt normal heart function due to its regulatory role in cardiac muscle contraction. Altered regulation within the cAMP and glycogen metabolism pathways can contribute to metabolic disorders like diabetes. Interactions with proteins such as PP1 and PKA are critical for these conditions highlighting PPP1R1A's role in disease progression and potential therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PPP1R1A antibody [EPR28192-90] (ab323837), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PPP1R1A antibody [EPR28192-90] (ab323837)

    PPP1R1A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse pancreas using rabbit Anti-PPP1R1A antibody

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling PPP1R1A with ab323837 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on islet of mouse pancreas.

    The primary antibody was incubated for 60 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PPP1R1A antibody [EPR28192-90] (ab323837), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PPP1R1A antibody [EPR28192-90] (ab323837)

    PPP1R1A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-PPP1R1A antibody

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PPP1R1A with ab323837 at 1/100 (5.0 ug/ml) dilution.

    Negative control: no staining on mouse liver.

    The primary antibody was incubated for 60 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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