JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB300097

Alexa Fluor® 488 Anti-Pro-Collagen III Alpha 1 antibody [EPR24255-13]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Collagen III antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF and reacts with Human samples.
2 Images
Immunohistochemistry - Alexa Fluor® 488 Anti-Pro-Collagen III Alpha 1 antibody [EPR24255-13] (AB300097)
  • IHC

Supplier Data

Immunohistochemistry - Alexa Fluor® 488 Anti-Pro-Collagen III Alpha 1 antibody [EPR24255-13] (AB300097)

Immunohistochemical analysis of paraffin-embedded Human cervical carci tissue labeling Pro-Collagen III alpha 1 with ab300097 at 1/50 (10.0 ug/ml) followed by a at dilution. Positive staining on the stromal cells of human cervical carcinoma. The section was incubated with ab300097 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI. Secondary antibody only control : Secondary antibody is at dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins

Immunohistochemistry - Alexa Fluor® 488 Anti-Pro-Collagen III Alpha 1 antibody [EPR24255-13] (AB300097)
  • IHC

Supplier Data

Immunohistochemistry - Alexa Fluor® 488 Anti-Pro-Collagen III Alpha 1 antibody [EPR24255-13] (AB300097)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Pro-Collagen III alpha 1 with ab300097 at 1/50 (10.0 ug/ml) followed by a at dilution. Positive staining on the stromal cells of human endometrium. The section was incubated with ab300097 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI. Secondary antibody only control : Secondary antibody is at dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins

  • Unconjugated

    Anti-Pro-Collagen III alpha 1 antibody [EPR24255-13]

  • Carrier free

    Anti-Pro-Collagen III alpha 1 antibody [EPR24255-13] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Pro-Collagen III alpha 1 antibody [EPR24255-13]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24255-13

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (AB222942)

  • 4
  • 1
Reagents, Controls & Accessories

AB246832

Picro-Sirius Red Solution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

  • 0
  • 0
Primary Antibodies

AB280968

Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (AB280968)

  • 1
  • 1
Primary Antibodies

AB198934

Alexa Fluor® 647 Anti-Fibronectin antibody [F1]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (AB198934)

  • 0
  • 0
Primary Antibodies

AB309502

Alexa Fluor® 488 Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Collagen IV antibody [EPR22911-127] (AB309502)

  • 5
  • 1
Primary Antibodies

AB313240

Alexa Fluor® 555 Anti-MyoD1 antibody [EPR6653-131]

RabMAb|Recombinant

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro-Collagen III alpha 1 also known as collagen III is a precursor to type III collagen. It has a molecular mass of approximately 139 kDa. This protein predominantly occurs in tissues like skin lung intestine and the vascular system where it plays a mechanical role by contributing to the structural integrity and mechanical properties of these organs. The synthesize beings in the fibroblasts and procollagen undergoes a series of post-translational modifications before it secreted into the extracellular matrix.
Biological function summary

This precursor form of collagen functions as part of the collagen formation process. As a component of the extracellular matrix it interacts with various components like glycosaminoglycans and elastin helping maintain elasticity and strength. Pro-collagen III is not a part of oligomeric complexes but rather after processing joins the fibrillar collagens to support tissue structure and repair.

Pathways

Pro-Collagen III alpha 1 plays a role in the processes of collagen biosynthesis and fibroblast growth signaling. It participates in the extracellular matrix organization pathway where it influences cellular adhesion migration and differentiation. This protein is related to other proteins such as collagen I and fibronectin through these pathways highlighting its importance in tissue integrity and wound healing.

The overproduction or dysfunction of Pro-Collagen III alpha 1 or its matured form collagen III often relates to fibrotic diseases and vascular conditions like Ehlers-Danlos syndrome. It interacts with collagen I in these scenarios which together can impact the phenotype of affected tissues leading to abnormalities in skin elasticity and cardiovascular complications. Understanding its role in these diseases helps in targeted therapies and potential treatment strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information COL3A1

Alternative Names

Collagen alpha-1(III) chain, COL3A1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com