Alexa Fluor® 488 Anti-Progesterone Receptor antibody [YR85]

4 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Progesterone Receptor antibody [YR85] (ab313737)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Progesterone Receptor with ab313737 at 1/100 (5.0 ug/ml) . Negative control: no staining on human cerebrum. The section was incubated with ab313737 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Progesterone Receptor antibody [YR85] (ab313737)

  Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Progesterone Receptor with ab313737 at 1/100 (5.0 ug/ml) . Nuclear staining on human endometrium. The section was incubated with ab313737 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Progesterone Receptor antibody [YR85] (ab313737)

  Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Progesterone Receptor with ab313737 at 1/100 (5.0 ug/ml) . Nuclear staining on human breast cancer. The section was incubated with ab313737 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Progesterone Receptor antibody [YR85] (ab313737)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling Progesterone Receptor with ab313737 at 1/50 (10 ug/ml) dilution(Green). Confocal image showing nuclear staining in T47D cells. Negative control: HepG2 (PMID:15297426). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).