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AB246777

Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal Prostaglandin E Receptor EP2/PTGER2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Prostaglandin E2 receptor EP2 subtype, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246777)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246777)

ab246777 staining Prostaglandin E Receptor EP2 in JAR cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab246777 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

  • Carrier free

    Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] - BSA and Azide free

  • Unconjugated

    Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

  • HRP

    HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8030(B)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB16151

Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [3E6]

Western blot - Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [3E6] (AB16151)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB237268

Alexa Fluor® 647 Anti-Heme Oxygenase 1 antibody [EPR18161-128]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB237268)

  • 0
  • 0
Primary Antibodies

AB217966

Anti-PGE2 receptor EP4 subtype antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

  • 0
  • 0
Primary Antibodies

AB252131

Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in Jar fixed with 4% formaldehyde (10 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Receptor EP2 also known as PTGER2 or EP2 receptor is a G-protein coupled receptor with a molecular mass of approximately 53 kDa. This receptor primarily binds prostaglandin E2 (PGE2) mediating various cellular responses. EP2 receptor is expressed in tissues such as the brain kidneys lungs and immune cells. Its activation leads to the stimulation of adenylyl cyclase increasing levels of cyclic AMP (cAMP) within the cell.
Biological function summary

The EP2 receptor plays a role in vasodilation immune modulation and regulation of pain and inflammation. It does not act as part of a large protein complex but rather exerts its effects individually through its coupling with G-proteins. EP2 receptor stimulation results in smooth muscle relaxation and has implications in neuroinflammation and renal function.

Pathways

The EP2 receptor is involved in the prostaglandin signaling pathway and contributes to the inflammatory response pathway. It shares a connection with other receptors such as EP1 EP3 and EP4 which also bind PGE2 but induce different cellular responses. These pathways integrate signals that modulate processes like inflammation and immune responses highlighting EP2's role in maintaining physiological balance.

EP2 receptor activity is associated with conditions like asthma and Alzheimer's disease. In asthma the receptor's activity may influence airway hyperresponsiveness through its effects on smooth muscle and immune cells. In Alzheimer's EP2 receptor-related pathways may contribute to neuroinflammation which is linked with disease progression. Connections with proteins like COX-2 can be noted in these disorders as COX-2-dependent prostaglandin synthesis directly affects EP2 receptor signaling and subsequent cellular outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
See full target information Prostaglandin E2 receptor EP2 subtype
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101380 PubMed38242120

2024

Surface CD52, CD84, and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Pettinella,Barbara Mariotti,Chiara Lattanzi,Kirsten Bruderek,Marta Donini,Sara Costa,Olivia Marini,Giulia Iannoto,Sara Gasperini,Elena Caveggion,Monica Castellucci,Federica Calzetti,Francisco Bianchetto-Aguilera,Elisa Gardiman,Matteo Giani,Stefano Dusi,Maurizio Cantini,Aurora Vassanelli,Denise Pavone,Michele Milella,Sara Pilotto,Pamela Biondani,Benedikt Höing,Marie Carolin Schleupner,Timon Hussain,Boris Hadaschik,Cordelia Kaspar,Carlo Visco,Cristina Tecchio,Leo Koenderman,Flavia Bazzoni,Nicola Tamassia,Sven Brandau,Marco A Cassatella,Patrizia Scapini
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com