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AB312894

Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806]

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Rabbit Recombinant Monoclonal Protein CASP antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

View Alternative Names

CUTL1, CUX1, Protein CASP

4 Images
Flow Cytometry - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labelling Protein CASP with ab312894 at 1/50 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling Protein CASP with ab312894 at 1/50 (10.0 μg/ml) dilution (Green). Confocal image showing cytoplasmic staining in C6 cells. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Protein CASP with ab312894 at 1/50 (10.0 μg/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Protein CASP antibody [EPR18806] (AB312894)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Protein CASP with ab312894 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18806

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein CASP also known as Caspase or CASP protein is an important player in the process of apoptosis. Caspases are cysteine-aspartic proteases that exist as inactive proenzymes made of a prodomain large and small subunits. Upon activation caspases cleave substrates after aspartic acid residues. The Caspase family proteins have similar structures with molecular masses around 20 kDa to 40 kDa. CASP proteins are widely expressed across different tissues in the body. Activation can happen through intrinsic and extrinsic pathways enabling them to mediate the orderly dismantling of cellular components.
Biological function summary

Protein CASP acts as an executioner enzyme in cell death. It forms part of intricate protein complexes notably the apoptosome which includes APAF1 and cytochrome c. Once activated CASP proteins initiate a cascade of proteolytic events that lead to the dismantling of the cell. This process is tightly regulated and is essential for maintaining homeostasis and tissue development. Other caspases work upstream to activate downstream executioner caspases further highlighting the protein's role in these biological functions.

Pathways

Protein CASP plays a significant role in apoptotic signaling pathways. It is pivotal in both the extrinsic and intrinsic apoptotic pathways. In the extrinsic pathway proteins such as FADD and DISC interact with CASP-8 to trigger apoptosis. The intrinsic pathway includes the release of cytochrome c which partners with APAF1 leading to the activation of CASP-9 and subsequently other caspases. This cascade illustrates the interconnected nature of apoptotic signaling with other molecular pathways ensuring the precise regulation of cell death.

Protein CASP is associated with cancer and neurodegenerative diseases. Dysregulation of CASP activity can result in either excessive cell death or survival contributing to disease pathogenesis. In cancers often CASP activity is impaired allowing cancer cells to evade apoptosis and proliferate unchecked. In neurodegenerative disorders such as Alzheimer's abnormal CASP activation contributes to neuronal loss. The interaction between CASP proteins and BCL-2 family members highlights their role in these diseases emphasizing the therapeutic targeting potential in modulating CASP activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in intra-Golgi retrograde transport.
See full target information CUX1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com