Alexa Fluor® 488 Anti-PTRF antibody [EPR27006-67]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PTRF antibody [EPR27006-67] (AB320701)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PTRF with ab320701 at 1/50 (10.0 ug/ml) dilution.

Positive staining on stromal cells of human kidney.
The section was incubated with ab320701 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PTRF antibody [EPR27006-67] (AB320701)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PTRF KO HeLa (PTRF knockout human cervical adenocarcinoma epithelial cells), ab265435 cells labelling PTRF with ab320701 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing membranous and cytoplasmic staining in wildtype HeLa cells and showing no staining in PTRF knockout HeLa cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PTRF antibody [EPR27006-67] (AB320701)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PTRF with ab320701 at 1/50 (10.0 ug/ml) dilution.

Positive staining on stromal cells of human ovarian carcinoma.
The section was incubated with ab320701 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-PTRF antibody [EPR27006-67] (AB320701)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling PTRF with ab320701 at 1/50 (10.0 ug/ml) dilution.

Positive staining on stromal cells of rat testis.
The section was incubated with ab320701 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins