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AB270094

Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

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(2 Publications)

Rabbit Recombinant Monoclonal RAB5A antibody - conjugated to Alexa Fluor® 488. Early Endosome marker. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

RAB5, RAB5A, Ras-related protein Rab-5A

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB270094)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB270094)

Flow cytometry overlay histogram showing wild-type Hap1 (green line) and RAB5A knockout Hap1 cells (ab) stained with ab270094 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab270094) (1x 106 in 100μL at 0.2μg/mL (1/2500) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) used at the same concentration and conditions as the primary antibody (wild-type Hap1 - black line RAB5A knockout Hap1- grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody can also be used in Hap1 cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB270094)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB270094)

ab270094 staining Rab5 in Hap1 (Human cell line) cells, with negative expression in Hap1-Rab5A cells.

The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270094 at 1/500 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • 578 PE

    PE Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 660 APC

    APC Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • HRP

    HRP Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Rab5 antibody [EPR21801]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • Unconjugated

    Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • Carrier free

    Anti-Rab5 antibody [EPR21801] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21801

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>This product gave a positive signal in Hap1 fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.
Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
See full target information RAB5A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1543657 PubMed40196735

2025

RAS70 peptide targets multiforme glioblastoma by binding to the plasma membrane heat shock protein HSP70.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim Shevtsov,Natalia Yudintceva,Danila Bobkov,Ruslana Likhomanova,Anastasiya Nechaeva,Elena Mikhailova,Elena Oganesyan,Viacheslav Fedorov,Andrey Kurkin,Anastasiya Lukacheva,Georgii Fofanov,Aleksander Kim,Evegeniy Fedorov,Daria Sitovskaya,Alexey Ulitin,Natalia Mikhailova,Ilya Anufriev,Maria Istomina,Ekaterina Murashko,Elizaveta Kessenikh,Nikolay Aksenov,Yulia Vakhitova,Konstantin Samochernykh,Emil Pitkin,Evgeny Shlyakhto,Stephanie E Combs

PLoS pathogens 18:e1010434 PubMed35390107

2022

Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ronni A G da Silva,Wei Hong Tay,Foo Kiong Ho,Frederick Reinhart Tanoto,Kelvin K L Chong,Pei Yi Choo,Alexander Ludwig,Kimberly A Kline
View all publications
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Product promise

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