Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

40 product images

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab199091 using various fixation and permeablisation. The cells were fixed, washed and permeablised as indicated below;
  

  4% formaldehyde (10 min, room temperature)/0.1% PBS-Triton X-100 (15 min, room temperature)

  80% methanol (5 min, -20°C)/0.1% PBS-Triton X-100 (15 min, room temperature)

  4% formaldehyde (10 min, room temperature)/90% methanol (30 min, -20°C)

  The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab199091) for 30 min at 22°C at the following concentrations - Blue line (Unlabelled), Black line (0.1μg/ml), Red line (1μg/ml) and Green line (10μg/ml) .

  Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] ab198978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] ab198978, 1/500 dilution) for 30 min at 22°C.
  

  Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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  Lin et al Adv Sci (Weinh). 2019 Jan 28;6(6):1802062. doi: 10.1002/advs.201802062. eCollection 2019 Mar 20. Fig S9.

  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
  

  CRT was measured using dual staining of PI and a monoclonal CRT antibody. Following 24 h after incubation, cells were washed with PBS, detached with 200 μL of accutase, and washed twice with 2 mL of FACS buffer (500 mL sheath fluid + 2 g bovine serum albumin + 1 g NaN₃ in 100 mL H₂O). Each sample was split into two vials and one was stained with monoclonal primary rabbit anti‐CRT antibody (Abcam, Alexa Fluor® 488 Anti-Calreticulin antibody [EPR3924] - ER Marker ab196158) while the other was stained with rabbit IgG, monoclonal isotype control (Abcam, ab199091) for 40 min at 4°C. Cells were then washed once with FACS buffer. 0.5 μL of PI was added to each sample immediately before being quantified with a flow cytometer. Fifteen thousand events were collected and only the PI− cells were analyzed for surface CRT expression. Data were analyzed and gated using the FlowJo software (FlowJo LLC, version 10). Data were expressed as percent CRT positive after accounting for nonspecific binding with their corresponding isotype.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199091 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse splenocytes cells labelling CD23 with Alexa Fluor® 488 Anti-CD23 antibody [EPR28712-26] ab322422 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Cells are co-stained with CD19 conjugated to PE/CY7.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of FOLR1 KO HeLa (human knockout cervical adenocarcinoma epithelial cell, Magenta) / Parental HeLa, Green) cells labelling Folate Binding Protein/FBP with Anti-Folate Binding Protein/FBP antibody [RM1246] ab322459 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with ab322943 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of A549 (human lung carcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling DARC with Alexa Fluor® 488 Anti-DARC antibody [EPR26544-110] ab318171 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Gated on viable cells.
  
  Negative control: A549.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse splenocytes cells labelling CD23 with Alexa Fluor® 488 Anti-CD23 antibody [EPR28712-26] ab322422 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of SK-MEL-28 (human malignant melanoma cell line) cells labelling CD63 with Alexa Fluor® 488 Anti-CD63 antibody [EPR22458-280] ab318237 at 1/5000 dilution(0.01ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Left panel: No fixation/permeabilization; Gated on viable cells for surface CD63 staining.
  
  Right panel: 4% paraformadehyde fixation / 90% methanol permeabilization.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD63 with Alexa Fluor® 488 Anti-CD63 antibody [EPR22458-280] ab318237 at 1/5000 dilution (0.01ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Gated on viable cells.
  
  Cells were stained with anti-CD14 conjugated to Alexa Fluor® 647.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse peripheral blood mononuclear cell (PBMC) treated with 500ng/ml ionomycin and 10ng/ml PMA for 24 hours cells labelling CD69 with Alexa Fluor® 488 Anti-CD69 antibody [EPR25398-81] ab322232 at 1/500 dilution (0.1ug) / Lower right and Upper right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were stained with anti-CD3 conjugated to Alexa Fluor® 647.
  
  Gated on viable cells.
  
  treated cells antibody: Lower right
  
  untreated cells antibody: Upper right
  
  treated cells isotype: Lower left
  
  untreated cells isotype: Upper left

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 500ng/ml ionomycin and 10ng/ml PMA for 3 hours cells labelling CD69 with Alexa Fluor® 488 Anti-CD69 antibody [EPR25398-81] ab322232 at 1/5000 dilution (0.01ug) / Lower right and Upper right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647.
  
  Gated on viable cells.
  
  treated cells antibody: Lower right
  
  untreated cells antibody: Upper right
  
  treated cells isotype: Lower left
  
  untreated cells isotype: Upper left

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] ab322240 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Parental HEK293T (Green) / PLOD2 KO HEK293T (human PLOD2 knockout embryonic kidney epithelial cell, Magenta) cells labelling PLOD2/LH2 with Alexa Fluor® 488 Anti-PLOD2/LH2 antibody [EPR25160-22] ab322320 at 1/20000 dilution (0.0025 ug), compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black and Grey) isotype control.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 with Alexa Fluor® 488 Anti-CD21 antibody [EPR27369-9] ab321857 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: K-562 (PMID: 6983911).
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] ab322240 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / SK-MEL-28 (human malignant melanoma, Right) cells labelling MiTF with Alexa Fluor® 488 Anti-MiTF antibody [EPR26363-10] ab322231 at 1/50000 dilution (0.001ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: MCF7 (PMID: 30651597).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse PBMC cells labelling Myeloperoxidase with Alexa Fluor® 488 Anti-Myeloperoxidase antibody [EPR17996] ab322168 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling IL-15 with Alexa Fluor® 488 Anti-IL-15 antibody [EPR22635-214] ab322228 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were surface stained with anti-CD11b conjugated to BV421. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or antibody.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (human cervical adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling ErbB3 / HER3 with Alexa Fluor® 488 Anti-ErbB3 / HER3 antibody [EPR22669-25] ab322172 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Low expression: Hela (PMID: 22436610).

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with Alexa Fluor® 488 Anti-NKR-P1C antibody [EPR22990-12] ab322225 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD19 conjugated to PE/CY7.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with Alexa Fluor® 488 Anti-NKR-P1C antibody [EPR22990-12] ab322225 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD49b conjugated to PE.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse spleenocytes cells labelling CLEC5A with Alexa Fluor® 488 Anti-CLEC5A antibody [EPR28104-14] ab321946 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Mouse splenocytes cells labelling CLEC5A with Alexa Fluor® 488 Anti-CLEC5A antibody [EPR28104-14] ab321946 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Parental Jurkat (Left) / CD1A KO Jurkat (CD1A knockout human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD1a with Alexa Fluor® 488 Anti-CD1a antibody [EPR26526-62] ab321942 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of CTLL-2 (mouse T lymphocyte, Left) / 3T3-L1 (mouse embryonic fibroblast, Right) cells labelling LTBR with Alexa Fluor® 488 Anti-LTBR antibody [EPR26095-87] ab321854 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: CTLL-2 (PMID: 9317127).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with Alexa Fluor® 488 Anti-CD21 antibody [EPR27369-9] ab321857 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD19 conjugated to PE/CY7.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with Alexa Fluor® 488 Anti-CD21 antibody [EPR27369-9] ab321857 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse bone marrow cells labelling Myeloperoxidase with Alexa Fluor® 488 Anti-Myeloperoxidase antibody [EPR17996] ab322168 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were stained with anti-Ly-6G conjugated to BV421. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or antibody.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CD1a with Alexa Fluor® 488 Anti-CD1a antibody [EPR26526-62] ab321942 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: HeLa.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CLEC5A with Alexa Fluor® 488 Anti-CLEC5A antibody [EPR28104-14] ab321946 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: EL4.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / KG-1a (human bone marrow myeloblast, Right) cells labelling CD34 with Alexa Fluor® 488 Anti-CD34 antibody [EPR27432-54] ab321858 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Daudi (PMID: 21286385, 10942240).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/ml Trichostatin A for another 4h (Green) /Untreated control (Magenta) cells labelling Histone H4 (acetyl K12) with Anti-Histone H4 (acetyl K12) antibody [EPR28340-173] ab320815 at 1/50 dilution (1ug) / magenta and green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell, Left) / U-937 (human histiocytic lymphoma monocyte, Right) cells labelling WASP/Wiskott-Aldrich syndrome protein with Alexa Fluor® 488 Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] ab320115 at 1/500 dilution (0.1ug) (Magenta) compared with a ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: A431 (PMID: 9207440).

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with Alexa Fluor® 488 Anti-CCR9 antibody [EPR26524-59] ab319184 at 1/500 dilution (0.1ug) (Red) compared with a ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Jurkat (PMID: 19525985).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with Myc-tagged ENPP3 overexpression construct (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling ENPP3/B10 with Alexa Fluor® 488 Anti-ENPP3/B10 antibody [EPR27349-72] ab319125 at 1/50 dilution (1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

  Cells were co-stained with anti-myc conjugated to Alexa Fluor 647.
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / KU812 (human Peripheral blood basophil, Right) cells labelling ENPP3/B10 with Alexa Fluor® 488 Anti-ENPP3/B10 antibody [EPR27349-72] ab319125 at 1/50 dilution (1ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

  Negative control: 293T (PMID: 11342463).
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16-F0 (mouse melanoma epithelial-like cell) cells labelling MiTF with Alexa Fluor® 488 Anti-MiTF antibody [EPR26363-10] ab322231 at 1/50000 dilution (0.001ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).