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Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt, Flow Cyt (Intra) and reacts with samples. Cited in 25 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

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Reactivity data

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/500

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/500

Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Application

Flow Cyt (Intra)

Reactivity

Reacts

Dilution info

1/500

Notes

-

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Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt, Flow Cyt (Intra) and reacts with samples. Cited in 25 publications.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Clone number

EPR25A

Purification technique

Affinity purification Protein A

Specificity

Please note Abcam have optimised the validation of this product. In our hands, we observe an increase in background signal intensity with the use of Triton X-100 and would recommend using an alternative permeabilisation method such as methanol or saponin.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

40 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab199091 using various fixation and permeablisation. The cells were fixed, washed and permeablised as indicated below;

    4% formaldehyde (10 min, room temperature)/0.1% PBS-Triton X-100 (15 min, room temperature)

    80% methanol (5 min, -20°C)/0.1% PBS-Triton X-100 (15 min, room temperature)

    4% formaldehyde (10 min, room temperature)/90% methanol (30 min, -20°C)

    The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab199091) for 30 min at 22°C at the following concentrations - Blue line (Unlabelled), Black line (0.1μg/ml), Red line (1μg/ml) and Green line (10μg/ml) .

    Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab198978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198978, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail
    Lin et al Adv Sci (Weinh). 2019 Jan 28;6(6):1802062. doi: 10.1002/advs.201802062. eCollection 2019 Mar 20. Fig S9.

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    CRT was measured using dual staining of PI and a monoclonal CRT antibody. Following 24 h after incubation, cells were washed with PBS, detached with 200 μL of accutase, and washed twice with 2 mL of FACS buffer (500 mL sheath fluid + 2 g bovine serum albumin + 1 g NaN3 in 100 mL H2O). Each sample was split into two vials and one was stained with monoclonal primary rabbit anti‐CRT antibody (Abcam, ab196158) while the other was stained with rabbit IgG, monoclonal isotype control (Abcam, ab199091) for 40 min at 4°C. Cells were then washed once with FACS buffer. 0.5 μL of PI was added to each sample immediately before being quantified with a flow cytometer. Fifteen thousand events were collected and only the PI− cells were analyzed for surface CRT expression. Data were analyzed and gated using the FlowJo software (FlowJo LLC, version 10). Data were expressed as percent CRT positive after accounting for nonspecific binding with their corresponding isotype.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199091 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab322240 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab322240 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 500ng/ml ionomycin and 10ng/ml PMA for 3 hours cells labelling CD69 with ab322232 at 1/5000 dilution (0.01ug) / Lower right and Upper right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    treated cells antibody: Lower right

    untreated cells antibody: Upper right

    treated cells isotype: Lower left

    untreated cells isotype: Upper left

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling IL-15 with ab322228 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells were surface stained with anti-CD11b conjugated to BV421. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or antibody.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse bone marrow cells labelling Myeloperoxidase with ab322168 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells were stained with anti-Ly-6G conjugated to BV421. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or antibody.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (human cervical adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling ErbB3 / HER3 with ab322172 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Low expression: Hela (PMID: 22436610).

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab322225 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD19 conjugated to PE/CY7.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab322225 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD49b conjugated to PE.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Mouse spleenocytes cells labelling CLEC5A with ab321946 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD11b conjugated to Brilliant Violet 421.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Mouse splenocytes cells labelling CLEC5A with ab321946 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with ab321857 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD19 conjugated to PE/CY7.

    Gated on viable cells.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse PBMC cells labelling Myeloperoxidase with ab322168 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A20 (mouse reticulum sarcoma B lymphocyte, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD68 with ab319086 at 1/5000 dilution (0.01ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Negative control: A20

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with ab321857 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD56 conjugated to Brilliant Violet 421.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CLEC5A with ab321946 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: EL4.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CD1a with ab321942 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: HeLa.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 with ab321857 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: K-562 (PMID: 6983911).

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / KG-1a (human bone marrow myeloblast, Right) cells labelling CD34 with ab321858 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: Daudi (PMID: 21286385, 10942240).

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/ml Trichostatin A for another 4h (Green) /Untreated control (Magenta) cells labelling Histone H4 (acetyl K12) with ab320815 at 1/50 dilution (1ug) / magenta and green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell, Left) / U-937 (human histiocytic lymphoma monocyte, Right) cells labelling WASP/Wiskott-Aldrich syndrome protein with ab320115 at 1/500 dilution (0.1ug) (Magenta) compared with a ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: A431 (PMID: 9207440).

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with ab319184 at 1/500 dilution (0.1ug) (Red) compared with a ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: Jurkat (PMID: 19525985).

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with Myc-tagged ENPP3 overexpression construct (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling ENPP3/B10 with ab319125 at 1/50 dilution (1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Cells were co-stained with anti-myc conjugated to Alexa Fluor 647.
    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / KU812 (human Peripheral blood basophil, Right) cells labelling ENPP3/B10 with ab319125 at 1/50 dilution (1ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Negative control: 293T (PMID: 11342463).
    Gated on viable cells.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / U266B1 (human multiple myeloma B lymphocyte, Right) cells labelling IgE with ab319124 at 1/5000 dilution (0.01ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Negative control: Jurkat.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Isotype control (Left) / Human peripheral blood mononuclear cell (PBMC) treated with 50ng/mL PMA and 500ng/mL Ionomycin for 24 hours (Middle) / Untreated control (Right) cells labelling LIGHT/TNFSF14 with ab319123 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Cells were stained with anti-CD56 conjugated to BV421.
    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Isotype control (Left) / Mouse peripheral blood mononuclear cell (PBMC) treated with 50ng/mL PMA and 500ng/mL Ionomycin for 24 hours (Middle) / Untreated control (Right) cells labelling LIGHT/TNFSF14 with ab319123 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Cells were stained with anti-CD3 conjugated to Alexa Fluor® 647.
    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of CTLL-2 (mouse T lymphocyte, Left) / 3T3-L1 (mouse embryonic fibroblast, Right) cells labelling LTBR with ab321854 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: CTLL-2 (PMID: 9317127).

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of THP-1 (human monocytic leukemia monocyte, Left) / HDLM-2 (human Hodgkin lymphoma cell, Right) cells labelling IL-18R1 with ab319087 at 1/50 dilution (1ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Negative control: THP-1 (PMID: 10865972).
    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Isotype (Left) / Human PBMC (human peripheral blood mononuclear cell) treated with 200 ng/ml IL-12 for 6 days (Right) cells labelling IL-18R1 with ab319087 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Cells co-stained with CD3 conjugated to BV421.
    Gated on viable cells.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged IL-23R expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty vector containing a Myc tag (Right) cells labelling IL-23R with ab319083 at 1/5000 dilution (0.01ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Live cells were surface stained with ab319083, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized AtT-20 (mouse pituitary tumor cell, Left) / Hepa1-6 (mouse hepatoma epithelial cell, Right) cells labelling PCSK9 with ab319077 at 1/500 dilution(0.1ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Low expression: AtT-20 (PMID: 28971102)

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer (NK-92®) cell with no GFP tag, Right) cells labelling NCAM1 with ab319016 at 1/500 dilution (0.1ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Negative control: MCF7 (PMID: 30050775).
    Gated on viable cells.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling pan-myc with ab319079 at 1/50 dilution (1ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling NCAM1 with ab319016 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

    Cells are co-stained with CD3 conjugated to BV421.
    Gated on viable cells.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NR8383 (rat alveolar macrophage) cells labelling CD68 with ab319086 at 1/5000 dilution (0.01ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

  • Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

    Flow cytometric analysis of Parental Jurkat (Left) / CD1A KO Jurkat (CD1A knockout human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD1a with ab321942 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

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