AB204650

Alexa Fluor® 488 Anti-RanGAP1 antibody [EPR3295]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RanGAP1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1835, SD, RANGAP1, Ran GTPase-activating protein 1, RanGAP1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RanGAP1 antibody [EPR3295] (AB204650)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RanGAP1 antibody [EPR3295] (AB204650)

ab204650 staining RanGAP1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204650 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-RanGAP1 antibody [EPR3295]
HRP

HRP Anti-RanGAP1 antibody [EPR3295]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3295

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanGAP1 also known as Ran GTPase-activating protein 1 plays an important role in regulating nucleocytoplasmic transport. Mechanically it converts RanGTP to RanGDP in the cytoplasm which is essential for the recycling of Ran and maintenance of the Ran gradient across the nuclear envelope. The molecular mass of RanGAP1 is approximately 70 kDa. RanGAP1 is expressed in various cell types with significant presence in the cytoplasm due to its function.

Biological function summary

Many cellular processes rely on the action of RanGAP1 as it forms part of the Ran cycle. This protein is an integral component of the nuclear transport complex which regulates the bidirectional movement of RNA and proteins through the nuclear pores. Through its action RanGAP1 indirectly influences nuclear import and export playing an essential part in cell cycle regulation and mitosis.

Pathways

Several key cellular pathways are dependent on RanGAP1 activity. For instance it is a component of the mitotic spindle assembly pathway and also plays a role in the RNA processing pathway. In these pathways RanGAP1 works closely with other proteins such as RanBP1 and RCC1 facilitating important cellular events that require strict regulation of nuclear-cytoplasmic transport.

Disruptions in RanGAP1 function have been associated with certain types of cancer and neurodegenerative diseases. Aberrant regulation of RanGAP1 activity can lead to improper cell cycle progression contributing to oncogenesis. Furthermore changes in the expression of RanGAP1 and its interacting proteins like RanBP2 may relate to disorders like Huntington's disease where cellular transport mechanisms are compromised.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase activator for RAN (PubMed : 16428860, PubMed : 8146159, PubMed : 8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed : 27160050, PubMed : 8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed : 27160050).

See full target information RANGAP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 37:19-38 PubMed29150432

2017

The IKK-related kinase TBK1 activates mTORC1 directly in response to growth factors and innate immune agonists.

Applications

Unspecified application

Species

Unspecified reactive species

Cagri Bodur,Dubek Kazyken,Kezhen Huang,Bilgen Ekim Ustunel,Kate A Siroky,Aaron Seth Tooley,Ian E Gonzalez,Daniel H Foley,Hugo A Acosta-Jaquez,Tammy M Barnes,Gabrielle K Steinl,Kae-Won Cho,Carey N Lumeng,Steven M Riddle,Martin G Myers,Diane C Fingar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-RanGAP1 antibody [EPR3295]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RanGAP1 antibody [EPR3295] (AB204650)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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