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AB290984

Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74]

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Rabbit Recombinant Monoclonal RBBP7 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC, WB and reacts with Mouse, Human samples.

View Alternative Names

RBAP46, RBBP7, Histone-binding protein RBBP7, Histone acetyltransferase type B subunit 2, Nucleosome-remodeling factor subunit RBAP46, Retinoblastoma-binding protein 7, Retinoblastoma-binding protein p46, RBBP-7

3 Images
Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (AB290984)
  • ICC

Unknown

Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (AB290984)

Immunofluorescent analysis of 4% Paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblasts) permeabilized with 0.1% TritonX-100 labelling RBBP7 with ab290984 at 1/50 dilution, (Green). Confocal image showing nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (AB290984)
  • ICC

Unknown

Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (AB290984)

Immunofluorescent analysis of 4% Paraformaldehyde fixed RBBP7 knockout human chronic myelogenous leukemia near-haploid cell line permeabilized with 0.1% TritonX-100 labelling RBBP7 with ab290984 at 1/50 dilution, (Green). Confocal image showing nuclear staining in Wild-type HAP1 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

Immunocytochemistry image showing no staining in RBBP7 knockout HAP1 cell line, and nuclear staining in wild type HAP1 cell line, which is specific.

Western blot - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (AB290984)
  • WB

Lab

Western blot - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (AB290984)

Blocking / diluent buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

False colour image of Western blot : Anti-RBBP7 antibody (ab290984) staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 loading control staining at 1/20000 dilution, shown in red.

All lanes:

Western blot - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984) at 1/500 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 50 kDa

false

Exposure time: 7s

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-RBBP7 antibody [EPR23796-74]

  • 578 PE

    PE Anti-RBBP7 antibody [EPR23796-74] (ChIP Grade)

  • 660 APC

    APC Anti-RBBP7 antibody [EPR23796-74] (ChIP Grade)

  • HRP

    HRP Anti-RBBP7 antibody [EPR23796-74] (ChIP Grade)

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-RBBP7 antibody [EPR23796-74] - ChIP Grade

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23796-74

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/50", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/50", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
See full target information RBBP7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com