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AB307864

Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y]

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Rabbit Recombinant Monoclonal ROCK2 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, p160 ROCK-1, ROCK-I, p160ROCK, ROCK1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling ROCK1 with ab307864 at 1/50 dilution (10.0 ug/ml). Positive staining on human ovarian carcinoma. The section was incubated with ab307864 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ROCK1 with ab307864 at 1/50 dilution (10.0 ug/ml). Positive staining on human placenta. The section was incubated with ab307864 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ROCK1 with ab307864 at 1/50 dilution (10.0 ug/ml). Positive staining on human testis. The section was incubated with ab307864 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ROCK1 antibody [EPR638Y] (AB307864)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling ROCK1 with ab307864 at 1/50 dilution (10.0 ug/ml). Negative control : no staining on human cardiac muscle. The section was incubated with ab307864 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR638Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed : 10436159, PubMed : 10652353, PubMed : 11018042, PubMed : 11283607, PubMed : 17158456, PubMed : 18573880, PubMed : 19131646, PubMed : 8617235, PubMed : 9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed : 10436159, PubMed : 10652353, PubMed : 11018042, PubMed : 11283607, PubMed : 17158456, PubMed : 18573880, PubMed : 19131646, PubMed : 23093407, PubMed : 23355470, PubMed : 8617235, PubMed : 9722579). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed : 18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed : 19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed : 19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed : 21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed : 19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).
See full target information ROCK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com