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AB205432

Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y]

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Rabbit Recombinant Monoclonal ROCK2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, p160 ROCK-1, ROCK-I, p160ROCK, ROCK1

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB205432)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB205432)

ab205432 staining ROCK2 + ROCK1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205432 at a 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Western blot - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB205432)
  • WB

Supplier Data

Western blot - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB205432)

This data was developed using ab45171, the same antibody clone in a different buffer formulation.

Loading control : Anti-6X His tag® antibody [EPR20547] (ab213204)

Blocking buffer and concentration : 5% NFDM/TBST

Exposure Times :

Lane 1 : 3 seconds

Lane 2 : 7 seconds

Lanes 1 - 2:

Western blot - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab205432) at 1/1000 dilution

Lanes 1 - 2:

Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (<a href='/en-us/products/primary-antibodies/pe-rock2-rock1-antibody-ep786y-ab224972'>ab224972</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (<a href='/en-us/products/primary-antibodies/rock2-rock1-antibody-ep786y-ab45171'>ab45171</a>) at 1/1000 dilution

Lane 1:

Recombinant Human ROCK1 protein (aa 1114 to 1354) (30 kDa)

Lane 2:

Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

  • Unconjugated

    Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 578 PE

    PE Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • Carrier free

    Anti-ROCK2 + ROCK1 antibody [EP786Y] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-ROCK1 antibody [EP786Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ROCK1 antibody [EP786Y]

  • 660 APC

    APC Anti-ROCK1 antibody [EP786Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-ROCK2 + ROCK1 antibody [EP786Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP786Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes both the cleaved C-terminus of ROCK 1 (30 kDa) and full length protein (158 kDa). The immunogen used for this product shares 83% homology with ROCK2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed : 10436159, PubMed : 10652353, PubMed : 11018042, PubMed : 11283607, PubMed : 17158456, PubMed : 18573880, PubMed : 19131646, PubMed : 8617235, PubMed : 9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed : 10436159, PubMed : 10652353, PubMed : 11018042, PubMed : 11283607, PubMed : 17158456, PubMed : 18573880, PubMed : 19131646, PubMed : 23093407, PubMed : 23355470, PubMed : 8617235, PubMed : 9722579). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed : 18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed : 19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed : 19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed : 21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed : 19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).
See full target information ROCK1

Additional targets

ROCK2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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