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AB225203

Alexa Fluor® 488 Anti-RPS10 antibody [EPR8545]

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(1 Publication)

Rabbit Recombinant Monoclonal RPS10 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Small ribosomal subunit protein eS10, 40S ribosomal protein S10, RPS10

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RPS10 antibody [EPR8545] (AB225203)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RPS10 antibody [EPR8545] (AB225203)

ab225203 staining RPS10 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225203 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-RPS10 antibody [EPR8545]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-RPS10 antibody [EPR8545]

  • 578 PE

    PE Anti-Ribosomal protein S10 antibody [EPR8545]

  • HRP

    HRP Anti-Ribosomal protein S10 antibody [EPR8545]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Ribosomal protein S10 antibody [EPR8545]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-RPS10 antibody [EPR8545]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-RPS10 antibody [EPR8545]

  • Carrier free

    Anti-RPS10 antibody [EPR8545] - BSA and Azide free

  • 660 APC

    APC Anti-Ribosomal protein S10 antibody [EPR8545]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Ribosomal protein S10 antibody [EPR8545]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8545

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS10 also known as 40S ribosomal protein S10 is a component of the small ribosomal subunit essential for protein synthesis. It weighs approximately 18.7 kDa. The protein is expressed in various tissues including the liver kidney and brain. Its primary role involves participating in the assembly and function of ribosomes by interacting with rRNA and supporting the translation of mRNA into amino acids.
Biological function summary

RPS10 contributes to the formation and stability of the ribosomal 40S subunit. It works as part of the ribosome complex important for initiating and propelling protein synthesis. This ribosomal complex performs a central role in cellular growth and proliferation as it aids in assembling polypeptide chains that proteins need for diverse cellular processes. By doing so it supports a wide range of physiological functions.

Pathways

RPS10 contributes to protein synthesis through the translational initiation and elongation pathways. Its participation supports the accurate decoding and synthesis of proteins which are critical to cellular metabolism and growth. RPS10 interacts with other ribosomal proteins such as RPS12 and RPS14 to maintain these pathways' function and efficiency helping regulate cellular homeostasis and response to environmental stimuli.

RPS10 mutations can link to Diamond-Blackfan Anemia a condition marked by failed red blood cell production impacting patient health. Moreover aberrations in RPS10 link to various cancers such as breast cancer due to its role in protein synthesis and cell growth. RPS10 interacts with proteins like RPL5 another ribosomal protein within these disease contexts suggesting a broader contribution of ribosomal dysfunction in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 40S ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).
See full target information 40S ribosomal protein S10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 23:1256-1272 PubMed35902638

2022

Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztian Csomos,Boglarka Ujhazi,Peter Blazso,Jose L Herrera,Christopher M Tipton,Tomoki Kawai,Sumai Gordon,Maryssa Ellison,Kevin Wu,Matthew Stowell,Lauren Haynes,Rachel Cruz,Bence Zakota,Johnny Nguyen,Michelle Altrich,Christoph B Geier,Svetlana Sharapova,Joseph F Dasso,Jennifer W Leiding,Grace Smith,Waleed Al-Herz,Mayra de Barros Dorna,Olajumoke Fadugba,Eva Fronkova,Veronika Kanderova,Michael Svaton,Sarah E Henrickson,Joseph D Hernandez,Taco Kuijpers,Snezhina Mihailova Kandilarova,Elizaveta Naumova,Tomas Milota,Anna Sediva,Despina Moshous,Benedicte Neven,Tara Saco,Ravishankar Sargur,Sinisa Savic,John Sleasman,Gauri Sunkersett,Brant R Ward,Masanobu Komatsu,Stefania Pittaluga,Attila Kumanovics,Manish J Butte,Michael P Cancro,Shiv Pillai,Eric Meffre,Luigi D Notarangelo,Jolan E Walter
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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