JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282161

Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SARS CoV-2 ORF3a Protein antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell line - SARS-CoV-2 samples.

View Alternative Names

ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB282161)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB282161)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized, HEK-293T (human embryonic kidney) transfected with myc-tagged SARS CoV-2 ORF3a. Protein expression construct (Right) stained with ab282161 at 1/500 dilution compared with HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-COV-1 ORF3a Protein expression construct (Left).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB282161)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9] (AB282161)

ab282161 staining SARS-CoV2 orf3a in 293T cells transfected with Myc-tagged SARS CoV-2 ORF3a protein expression vector cells, with negative expression in 293T cells transfected with Myc-Tag cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab282161 at 10 μg/ml (shown in green) and Myc-Tag Mouse mAb (Alexa Fluor® 647) at 2.5 μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24848-9

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

SARS-CoV-2

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 ORF3a protein also known as ORF3 is an accessory protein of the SARS-CoV-2 virus weighing approximately 275 amino acids. It is expressed in infected host cells and plays an important role in the virus's lifecycle. ORF3a is known for its role as an ion channel involved in the viral replication cycle. Its functions include altering host cell processes to facilitate viral propagation and possibly contributing to viral pathogenesis.
Biological function summary

The SARS-CoV-2 ORF3a protein interferes with the host immune response promoting virus survival and replication. ORF3a is implicated in the enhancement of viral pathogenic effects by modulating innate and adaptive immunity. Though not part of a large known stable complex ORF3a interacts with other viral and host proteins impacting processes like apoptosis and inflammation.

Pathways

ORF3a affects several critical processes within the host cell. It plays a role in the apoptosis pathway influencing cell death mechanisms that help the virus evade immune defenses. Additionally it modulates the NF-kB signaling pathway which is vital for immune response regulation. Other related proteins include ORF7a and NSP6 which work alongside ORF3a to manipulate host cell environments to the virus's advantage.

The SARS-CoV-2 ORF3a protein is directly connected to COVID-19 impacting the severity and progression of the disease. Its interaction with host proteins contributes to dysregulated immune responses and cytokine storms characteristic of severe COVID-19 cases. The protein's role in apoptosis links it to potential lung tissue damage exacerbating respiratory conditions associated with COVID-19 infections.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in viral egress via lysosomal trafficking (PubMed : 33157038, PubMed : 33422265). Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking (PubMed : 33157038, PubMed : 33422265, PubMed : 34158638). Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes (PubMed : 33422265). This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress (PubMed : 33422265). Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1 (PubMed : 35239449). This induces endoplasmic reticulum stress and inflammatory responses and facilitates viral infection (PubMed : 35239449).
See full target information 3a

Additional targets

SARS CoV-2 ORF3a Protein
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB283243

Alexa Fluor® 488 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (AB283243)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB283943

Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - BSA and Azide free

RabMAb|Recombinant

ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - BSA and Azide free (AB283943)

  • 0
  • 0
Primary Antibodies

AB284040

Anti-SARS-CoV-2 orf3d antibody [EPR25045-51]

RabMAb|20ul selling size|Recombinant

Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)

  • 1
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com