Alexa Fluor® 488 Anti-SCD1 antibody [EPR21963]

5 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SCD1 antibody [EPR21963] (ab315182)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling SCD1 with ab315182 at 1/100 (5.0 ug/ml).

  Negative control: no staining on human skeletal muscle. The section was incubated with ab315182 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SCD1 antibody [EPR21963] (ab315182)

  Immunohistochemical analysis of paraffin-embedded Rat adipose tissue labeling SCD1 with ab315182 at 1/100 (5.0 ug/ml).

  Cytoplasmic staining on rat adipose of colon. The section was incubated with ab315182 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SCD1 antibody [EPR21963] (ab315182)

  Immunohistochemical analysis of paraffin-embedded Mouse adipose tissue labeling SCD1 with ab315182 at 1/100 (5.0 ug/ml).

  Cytoplasmic staining on mouse adipose of pancreas. The section was incubated with ab315182 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SCD1 antibody [EPR21963] (ab315182)

  Immunohistochemical analysis of paraffin-embedded Human adipose tissue labeling SCD1 with ab315182 at 1/100 (5.0 ug/ml).

  Cytoplasmic staining on human adipose of stomach. The section was incubated with ab315182 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SCD1 antibody [EPR21963] (ab315182)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SCD1 with ab315182 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).