Mouse Monoclonal SCP3 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 6 publications.
Images
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (AB205846), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-scp3-antibody-cor-10g11-7-ab205846--immunohistochemistry-frozen-sections-img107386.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (AB205846)")
Publications
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- Development (Cambridge, England) 149:2022Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOleksandr Kirsanov,Taylor Johnson,Taylor Malachowski,Bryan A Niedenberger,Emma A Gilbert,Debajit Bhowmick,P Hande Ozdinler,Douglas A Gray,Kelsey Fisher-Wellman,Brian P Hermann,Christopher B GeyerPubMed 36250451
- PLoS genetics 18:e10104162022The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatoya J Peart,Taylor A Johnson,Sungkyoung Lee,Matthew J Sears,Fang Yang,Mathieu Quesnel-Vallières,Huijuan Feng,Yocelyn Recinos,Yoseph Barash,Chaolin Zhang,Brian P Hermann,P Jeremy Wang,Christopher B Geyer,Russ P CarstensPubMed 36129965
- Nature communications 13:45102022PRC1-mediated epigenetic programming is required to generate the ovarian reserve.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengwen Hu,Yu-Han Yeh,Yasuhisa Munakata,Hironori Abe,Akihiko Sakashita,So Maezawa,Miguel Vidal,Haruhiko Koseki,Neil Hunter,Richard M Schultz,Satoshi H NamekawaPubMed 35948547
- Communications biology 4:13712021Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumit Sandhu,Ieng F Sou,Jill E Hunter,Lucy Salmon,Caroline L Wilson,Neil D Perkins,Neil Hunter,Owen R Davies,Urszula L McClurgPubMed 34880391
- Journal of visualized experiments : JoVE :2021Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Han Yeh,Mengwen Hu,Toshinori Nakagawa,Akihiko Sakashita,Shosei Yoshida,So Maezawa,Satoshi H NamekawaPubMed 33522502
- Current biology : CB 30:408-420.e52020The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHironori Abe et. al.PubMed 31902729
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | |
|---|---|
| Mouse | Tested |
| Hamster | Predicted |
| Zebrafish | Predicted |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes This product gave a positive signal in mouse frozen testis tissue fixed with 10% formaldehyde (10 min). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed:11311943, PubMed:22761579). Required for centromere pairing during meiosis in male germ cells (PubMed:22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed:10678170). Plays a lesser role in female fertility (PubMed:10678170, PubMed:12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed:22346761).
Alternative names
Scp3, Sycp3, Synaptonemal complex protein 3, SCP-3
Recommended products
Mouse Monoclonal SCP3 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 6 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Cor 10G11/7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.
- Biological function summary
SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.
- Pathways
SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.
- Associated diseases and disorders
SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-scp3-antibody-cor-10g11-7-ab205846--immunohistochemistry-frozen-sections-img107386.jpg "Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)")
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)
IHC image of SCP3 staining in a section of frozen normal mouse testis.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab205846 at 1/100 (shown in green) and counterstained using Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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