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AB205846

Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7]

5

(1 Review)

|

(12 Publications)

Mouse Monoclonal SCP3 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 12 publications.

View Alternative Names

Scp3, Sycp3, Synaptonemal complex protein 3, SCP-3

1 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (AB205846)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (AB205846)

Immunofluorescence staining of SCP3 in a section of frozen normal mouse testis.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND™. The section was incubated at room temperature for 1 hour with ab205846 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Cor 10G11/7

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>This product gave a positive signal in mouse frozen testis tissue fixed with 10% formaldehyde (10 min).</p>" }, "Hamster": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Zebrafish": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.
Biological function summary

SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.

Pathways

SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.

SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed : 11311943, PubMed : 22761579). Required for centromere pairing during meiosis in male germ cells (PubMed : 22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed : 10678170). Plays a lesser role in female fertility (PubMed : 10678170, PubMed : 12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed : 22346761).
See full target information Sycp3
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:63 PubMed39747485

2025

Cep78 knockout causes sterility and oligoasthenoteratozoospermia in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min Liu,Zongzhuang Wen,Dapeng Zhao,Wei Tian,Qingfeng Lv,Chunling Zhang,Xueyan Zhang,Fengling Meng,Hui Liu,Jiangang Gao,Zhiwei Yao

eLife 12: PubMed38226689

2024

Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Steven A Cincotta,Nainoa Richardson,Mariko H Foecke,Diana J Laird

Development (Cambridge, England) 150: PubMed37350382

2023

Retinoic acid is dispensable for meiotic initiation but required for spermiogenesis in the mammalian testis.

Applications

Unspecified application

Species

Unspecified reactive species

Oleksandr Kirsanov,Taylor A Johnson,Bryan A Niedenberger,Taylor N Malachowski,Benjamin J Hale,Qing Chen,Brad Lackford,Jiajia Wang,Anukriti Singh,Karen Schindler,Brian P Hermann,Guang Hu,Christopher B Geyer

Biology of reproduction 108:822-836 PubMed36708226

2023

Differential responsiveness of spermatogonia to retinoic acid dictates precocious differentiation but not meiotic entry during steady-state spermatogenesis†.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor A Johnson,Bryan A Niedenberger,Oleksandr Kirsanov,Ellen V Harrington,Taylor Malachowski,Christopher B Geyer

Development (Cambridge, England) 149: PubMed36250451

2022

Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Oleksandr Kirsanov,Taylor Johnson,Taylor Malachowski,Bryan A Niedenberger,Emma A Gilbert,Debajit Bhowmick,P Hande Ozdinler,Douglas A Gray,Kelsey Fisher-Wellman,Brian P Hermann,Christopher B Geyer

PLoS genetics 18:e1010416 PubMed36129965

2022

The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Natoya J Peart,Taylor A Johnson,Sungkyoung Lee,Matthew J Sears,Fang Yang,Mathieu Quesnel-Vallières,Huijuan Feng,Yocelyn Recinos,Yoseph Barash,Chaolin Zhang,Brian P Hermann,P Jeremy Wang,Christopher B Geyer,Russ P Carstens

Nature communications 13:4510 PubMed35948547

2022

PRC1-mediated epigenetic programming is required to generate the ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Mengwen Hu,Yu-Han Yeh,Yasuhisa Munakata,Hironori Abe,Akihiko Sakashita,So Maezawa,Miguel Vidal,Haruhiko Koseki,Neil Hunter,Richard M Schultz,Satoshi H Namekawa

Acta biochimica Polonica 69:79-84 PubMed35232009

2022

Cervical cancer cell proliferation inhibition by vanillin oxime through HIF‑1α expression inhibition, ERK1/2 and Akt protein down-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Jing Chen,Nai-Ling Li,Qiang Gu,Guang-Min Zhang

Communications biology 4:1371 PubMed34880391

2021

Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12.

Applications

Unspecified application

Species

Unspecified reactive species

Sumit Sandhu,Ieng F Sou,Jill E Hunter,Lucy Salmon,Caroline L Wilson,Neil D Perkins,Neil Hunter,Owen R Davies,Urszula L McClurg

Biology of reproduction 105:1603-1616 PubMed34518881

2021

Aurora A Kinase (AURKA) is required for male germline maintenance and regulates sperm motility in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

William C Lester,Taylor Johnson,Ben Hale,Nicholas Serra,Brian Elgart,Rong Wang,Christopher B Geyer,Ann O Sperry
View all publications
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