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Mouse Monoclonal SDHA antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Cow
Predicted
Predicted
Dog
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
0.2 µg/mL
Notes

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat, Cow, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Cow, Dog
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

2 products for Alternative Version

Target data

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).

Alternative names

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

Recommended products

Mouse Monoclonal SDHA antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
2E3GC12FB2AE2
Purity
IgG fraction
Concentration
Loading...
Purification notes

Purity is near homogeneity as judged by SDS-PAGE. ab154473 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Associated diseases and disorders

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

Product promise

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4 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

    ab154473 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab154473 at 1/1000 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

    Flow Cytometry for Anti-SDHA Alexa-488 antibody (ab154473) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL anti-SDHA Alexa-488 (red) stained cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

    Immunocytochemistry with HeLa cells (100x) were stained with anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green and DAPI in blue, as a nuclear stain.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

    Immunocytochemistry with HDFn (100x) cells were stained with Anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green, Anti-HSP60 (1/1000, Anti-Hsp60 antibody - Mitochondrial Marker ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).

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