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AB282658

Alexa Fluor® 488 Anti-SEC23B antibody [EPR22723-24]

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Rabbit Recombinant Monoclonal SEC23B antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, IHC-P and reacts with Mouse, Human samples.

View Alternative Names

Protein transport protein Sec23B, hSec23B, SEC23-related protein B, SEC23B

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SEC23B antibody [EPR22723-24] (AB282658)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SEC23B antibody [EPR22723-24] (AB282658)

ab282658 staining SEC23B in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab282658 at 1/500 dilution (shown in green) and ab190573, Rabbit monoclonal to alphaTubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SEC23B antibody [EPR22723-24] (AB282658)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SEC23B antibody [EPR22723-24] (AB282658)

Immunofluorescence staining of SEC23B staining in a section of formalin-fixed paraffin-embedded mouse normal liver.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab282658 at 1/50 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22723-24

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>This product gave a positive signal in Raw264.7 fixed with 100% methanol (5 min).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>This product gave a positive signal in Raw264.7 fixed with 100% methanol (5 min).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC23B also known as Protein Transport Protein Sec23B is a component of the coat protein complex II (COPII) which mediates transport from the endoplasmic reticulum to the Golgi apparatus. SEC23B weighs approximately 88 kDa and acts as a GTPase activating protein that regulates the COPII complex assembly. SEC23B broadly expresses in many tissues including high levels in the pancreas and liver suggesting its essential role in cellular transport processes.
Biological function summary

SEC23B functions in vesicular trafficking within cells. In the COPII complex it interacts with SEC24 proteins to form a structural framework critical for cargo selection and vesicle budding. SEC23B does not work alone and is part of a larger protein complex that includes SEC24 SAR1 and SEC13/31 which facilitates proper vesicle formation and trafficking. These interactions enable cells to accurately and efficiently transport necessary proteins and lipids through the secretory pathway.

Pathways

SEC23B is essential in the ER-to-Golgi transport pathway and plays a significant role in maintaining protein homeostasis. The secretory pathway requires SEC23B to cooperate with other proteins like SEC24C and SEC31A ensuring that cargo proteins are correctly packaged and delivered to their destination within the cell. This process is vital for the function of other pathways such as the unfolded protein response as it prevents the accumulation of misfolded proteins in the endoplasmic reticulum.

SEC23B mutations have links to Congenital Dyserythropoietic Anemia type II (CDA II) which manifests as ineffective erythropoiesis and improper glycosylation of red blood cell membrane proteins. SEC23B is also associated with pancreatic dysfunctions considering its high expression in the tissue although detailed mechanisms are less understood. In CDA II SEC23B's dysfunction affects its interaction with associated proteins like SEC24B disrupting normal vesicular trafficking and functional erythrocyte production.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.
See full target information SEC23B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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