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AB319553

Alexa Fluor® 488 Anti-SEC62 antibody [EPR9213]

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Rabbit Recombinant Monoclonal SEC62 antibody - conjugated to Alexa Fluor® 488.

View Alternative Names

TLOC1, SEC62, Translocation protein SEC62, Translocation protein 1, TP-1, hTP-1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9213

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC62 also known as translocation protein SEC62 is an essential component of the protein translocation machinery in cells. It is part of the Sec61 complex and helps with the translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane. SEC62 has a molecular mass of approximately 24 kilodaltons. It is expressed widely in tissues with higher expression levels observed in the pancreas and liver which are essential for protein synthesis and secretion.
Biological function summary

Beyond its role in the ER SEC62 contributes to the maintenance of calcium homeostasis and cellular stress responses. It forms a part of the SEC63 complex working alongside SEC63 and SEC61 to facilitate protein folding and assembly. This protein also plays a role in the response to ER stress where it assists in relieving the burden by managing calcium leakage across the ER membrane which is important for maintaining cell health under stress conditions.

Pathways

SEC62 plays a significant role in the signal recognition particle (SRP) pathway and the unfolded protein response (UPR). It interacts with other proteins in these pathways including SEC63 and BIP to regulate protein processing and transport. The SEC61 complex which includes SEC62 integrates signals for targeting nascent proteins to the ER membrane and aids in managing misfolded protein accumulation through UPR therefore ensuring cellular homeostasis.

SEC62 has been linked to various types of cancer including prostate and thyroid cancer where its overexpression correlates with tumor progression and metastasis. It interacts with proteins like BIP in cancer cells contributing to the ER stress response that supports cancer cell survival. Additionally alterations in SEC62 are associated with congenital disorders like congenital disorders of glycosylation (CDG) where dysfunctional protein translocation and glycosylation processes impact cellular function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates post-translational transport of precursor polypeptides across endoplasmic reticulum (ER). Proposed to act as a targeting receptor for small presecretory proteins containing short and apolar signal peptides. Targets and properly positions newly synthesized presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen.
See full target information SEC62
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com