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AB275185

Alexa Fluor® 488 Anti-Serpin A5 antibody [EPR23337-112]

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Rabbit Recombinant Monoclonal Serpin A5 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

PCI, PLANH3, PROCI, SERPINA5, Plasma serine protease inhibitor, Acrosomal serine protease inhibitor, Plasminogen activator inhibitor 3, Protein C inhibitor, Serpin A5, PAI-3, PAI3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Serpin A5 antibody [EPR23337-112] (AB275185)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Serpin A5 antibody [EPR23337-112] (AB275185)

Immunofluorescence staining of Serpin A5 staining in a section of formalin-fixed paraffin-embedded human normal kidney*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab275185 at 1/100 dilution (shown in green) and counterstained using ab190573, Rabbit anti-Tubulin (A647)(1/250) at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Unconjugated

    Anti-Serpin A5 antibody [EPR23337-112]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Serpin A5 antibody [EPR23337-112]

  • Carrier free

    Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23337-112

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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AB277462

Human Serpin A5 ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serpin A5 also known as Protein C Inhibitor (PCI) or Mesotrypsin inhibitor is a member of the serine protease inhibitor family with a mass of approximately 47 kDa. It plays a mechanical role as an inhibitor of several proteases such as trypsin tissue-type plasminogen activator and urokinase-type plasminogen activator. This protein is highly expressed in tissues including the liver kidneys and seminal plasma suggesting its involvement in reproductive and hemostatic processes.
Biological function summary

Serpin A5 regulates critical physiological processes through its inhibitory function. It interacts with and inhibits target proteases controlling pathways involved in coagulation and fibrinolysis. Serpin A5 does not form part of larger protein complexes but exerts its function through direct interactions. Its modulatory action impacts processes such as cell migration and tumor invasion highlighting its role in broader cellular environments.

Pathways

Serpin A5 is an important regulator in the coagulation cascade and fibrinolytic pathways. It acts to modulate the balance between coagulation and fibrinolysis critical for maintaining hemostasis. This protein is related to other serpins such as Antithrombin III and Plasminogen activator inhibitor-1 through its regulatory actions in these pathways. The inhibition of proteases by Serpin A5 ensures proper activation and termination of these biological processes which are essential for vascular integrity.

Serpin A5 is connected to conditions like thrombophilia and prostate cancer. Dysregulation in its expression or function leads to altered coagulation increasing the risk of thrombotic events. In prostate cancer its role is linked through pathways involving the matrix metalloproteinases where Serpin A5 can influence tumor progression and metastasis. Understanding its interactions with proteins in these diseases provides insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and pro-inflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue- and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.
See full target information SERPINA5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Version
Primary Antibodies

AB275186

Alexa Fluor® 647 Anti-Serpin A5 antibody [EPR23337-112]

primary-antibodies

alexa-fluor-647-serpin-a5-antibody-epr23337-112-ab275186

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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