AB323761

Alexa Fluor® 488 Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83]

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal SIGLEC10 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

SLG2, UNQ477/PRO940, SIGLEC10, Sialic acid-binding Ig-like lectin 10, Siglec-10, Siglec-like protein 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] (AB323761)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] (AB323761)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a SIGLEC10 expression vector containing a Myc-His tag. (B) HEK-293T transfected with a SIGLEC11 expression vector containing a Myc-His tag. (C) HEK-293T transfected with a SIGLEC16 expression vector containing a Myc-His tag. (D) HEK-293T transfected with a SIGLEC5 expression vector containing a Myc-His tag. (E) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling SIGLEC10 + SIGLEC11 with ab323761 at 1/100 (5.0 ug/ml) dilution.

This antibody recognizes both SIGLEC10 and SIGLEC11. Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a SIGLEC10 expression vector containing a Myc-His tag and (B) HEK-293T transfected with a SIGLEC11 expression vector containing a Myc-His tag, negative staining on (C) HEK-293T transfected with a SIGLEC16 expression vector containing a Myc-His tag, (D) HEK-293T transfected with a SIGLEC5 expression vector containing a Myc-His tag and (E) HEK-293T transfected with empty vector containing a Myc-His tag.
The section was incubated with ab323761 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] (AB323761)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SIGLEC10 + SIGLEC11 with ab323761 at 1/100 (5.0 ug/ml) dilution.

Low expression : nearly no staining on human skeletal muscle.
The section was incubated with ab323761 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] (AB323761)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SIGLEC10 + SIGLEC11 with ab323761 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human tonsil.
The section was incubated with ab323761 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29036-83

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

WB signal is only observed in tissue lysates.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC10 and SIGLEC11 are members of the sialic acid-binding immunoglobulin-type lectins family also known as siglecs. SIGLEC10 sometimes called CD33L1 has an approximate molecular mass of 85 kDa. SIGLEC11 has a molecular mass of about 101 kDa. These proteins are transmembrane receptors involved in cellular signaling. SIGLEC10 is expressed in various tissues including the placenta and spleen while SIGLEC11 expression is found mainly in the microglia of the brain. Their primary role is to mediate interactions with sialylated glycan ligands which affects cell-cell communication.

Biological function summary

SIGLEC10 and SIGLEC11 play important roles in the immune response by regulating inflammatory processes and helping the body distinguish between self and non-self cells. They function as part of the immune system to modulate immune cell activation damping down potentially harmful overreactions. These proteins can inhibit immune cell signaling when they engage with sialic acid-containing structures representing a form of immune checkpoint control. Their involvement in dampening inflammatory responses makes them part of the innate immune complex important in maintaining tissue homeostasis.

Pathways

SIGLEC10 and SIGLEC11 both interact with immunoregulatory pathways like the CD33-related and B cell receptor signaling pathways. Through these pathways they are involved in controlling immune responses and maintaining cellular communication balance. SIGLEC10 shows relationship with proteins such as SHP-1 and SHP-2 which are phosphatases that play critical roles in signal transduction. SIGLEC11 however interacts within microglial function pathways involving proteins associated with the innate immune response.

SIGLEC10 and SIGLEC11 connect to conditions such as Alzheimer's disease and rheumatoid arthritis. In AlzheimerЎЇs SIGLEC11 interaction with microglia influences the disease's neuroinflammatory component. Its role can help modulate the immune environment in the brain influencing disease progression. SIGLEC10ЎЇs dysfunctional regulation may contribute to the chronic inflammation observed in rheumatoid arthritis often mediated by aberrant immune responses. This relation involves other siglec family members like SIGLEC9 which together modulate inflammatory pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, seems to act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules (PubMed : 11284738, PubMed : 12163025). Involved in negative regulation of B-cell antigen receptor signaling. The inhibition of B cell activation is dependent on PTPN6/SHP-1 (By similarity). In association with CD24 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90 (By similarity). In association with CD24 may regulate the immune repsonse of natural killer (NK) cells (PubMed : 25450598). Plays a role in the control of autoimmunity (By similarity). During initiation of adaptive immune responses by CD8-alpha(+) dendritic cells inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes. The function seems to implicate recruitment of PTPN6/SHP-1, which dephosphorylates NCF1 of the NADPH oxidase complex consequently promoting phagosomal acidification (By similarity).

See full target information SIGLEC10

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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