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Rabbit Recombinant Monoclonal SUCLA2 antibody - conjugated to Alexa Fluor® 488.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed:15877282). The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SUCLA2 antibody - conjugated to Alexa Fluor® 488.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR14924

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SUCLA2 also known as succinate-CoA ligase (ADP-forming) subunit beta is an enzyme with a molecular mass of approximately 48 kDa. It plays an important role in the mitochondrial matrix where it participates in the citric acid cycle. The enzyme is found in various tissues with higher expression in muscle and brain tissues. SUCLA2 functions as part of the succinyl-CoA synthetase complex which catalyzes the reversible conversion of succinyl-CoA to succinate with the concurrent substrate-level phosphorylation of GDP to GTP or ADP to ATP depending on tissue type.

Biological function summary

The SUCLA2 subunit is essential for energy metabolism. It forms a component of the succinyl-CoA synthetase complex working with the alpha subunit SUCLG1. This complex supports the provision of energy by converting GDP to GTP in processes needing high energy levels critical for maintaining cellular homeostasis. Therefore it especially supports tissues with high energy demands. The action of SUCLA2 links energy production with the metabolic pathways involved in cellular respiration.

Pathways

SUCLA2 plays a critical role in the citric acid cycle also known as the Krebs cycle. Part of this cycle SUCLA2 contributes to cellular respiration and energy production. The SUCLA2 protein closely interacts with SUCLG1 to form the functional succinyl-CoA synthetase enzyme. Additionally it shows connections to the electron transport chain where the products of its catalysis are used in ATP generation. Defects in these pathways can have widespread effects on cellular energy states and mitochondrial function.

Associated diseases and disorders

SUCLA2 mutations are linked to mitochondrial DNA depletion syndromes particularly myopathy and encephalopathy. These conditions lead to severe energy metabolism impairments due to faulty mitochondrial function. SUCLA2 has also been associated with Leigh syndrome a neurodegenerative disorder marked by compromised cellular respiration. Such disorders point towards deficient ATP synthesis highlighting the interconnected role of SUCLA2 with other mitochondrial proteins involved in cellular energy metabolism.

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