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AB205775

Alexa Fluor® 488 Anti-TAF15 antibody [EPR9197(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal TAF15 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RBP56, TAF2N, TAF15, TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, RNA-binding protein 56, TAF(II)68, TAFII68

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TAF15 antibody [EPR9197(B)] (AB205775)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TAF15 antibody [EPR9197(B)] (AB205775)

ab205775 staining TAF15 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205775 at a 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TAF15 antibody [EPR9197(B)]

  • 578 PE

    PE Anti-TAF15 antibody [EPR9197(B)]

  • 660 APC

    APC Anti-TAF15 antibody [EPR9197(B)]

  • HRP

    HRP Anti-TAF15 antibody [EPR9197(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TAF15 antibody [EPR9197(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TAF15 antibody [EPR9197(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-TAF15 antibody [EPR9197(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-TAF15 antibody [EPR9197(B)]

  • Unconjugated

    Anti-TAF15 antibody [EPR9197(B)]

  • Carrier free

    Anti-TAF15 antibody [EPR9197(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9197(B)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Anti-TAF15 antibody [EPR9196(B)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

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Proteins & Peptides

AB174418

Recombinant Human TAF15 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TAF15 protein (His tag N-Terminus) (AB174418)

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Primary Antibodies

AB201020

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF15 also known as TATA-binding protein-associated factor 15 is a RNA polymerase II-associated factor with approximate mass of 56 kDa. It belongs to the FET family of proteins which include FUS and EWS. This protein expresses widely in various tissues including the brain and immune cells. Mechanically TAF15 participates in transcription by mediating interactions between transcription factors and the RNA polymerase II complex.
Biological function summary

The protein helps regulate transcription initiation and elongation. TAF15 operates as a part of the transcription pre-initiation complex working closely with other transcription-associated factors to control gene expression. Its role extends beyond the nucleus as it may participate in RNA processing and transport due to its ability to bind RNA.

Pathways

TAF15 plays a role in the regulation of transcription from RNA polymerase II promoters. It interacts with numerous other proteins such as TFIID and RNA polymerase II subunits facilitating transcriptional regulation. TAF15 also associates with pathways related to stress response and cellular growth emphasizing its role in maintaining cellular integrity and function.

TAF15 links to certain forms of cancer and neurodegenerative diseases. Its misregulation or mutation associates with some sarcomas indicating a role in tumorigenesis possibly through interactions with oncogenic proteins like EWS. In neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) TAF15 might be implicated potentially through pathways shared with proteins like TDP-43 that are involved in RNA metabolism dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
See full target information TAF15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 23:631-641 PubMed34108659

2021

Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda G Kugeratski,Kelly Hodge,Sergio Lilla,Kathleen M McAndrews,Xunian Zhou,Rosa F Hwang,Sara Zanivan,Raghu Kalluri
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com