JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317594

Alexa Fluor® 488 Anti-Tau Alzheimer's Disease antibody [GT-38] - Conformation-Specific

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal TAU antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr and reacts with Human samples.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Big tau

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Tau Alzheimer's Disease antibody [GT-38] - Conformation-Specific (AB317594)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Tau Alzheimer's Disease antibody [GT-38] - Conformation-Specific (AB317594)

Negative immunofluorescence staining of Tau Alzheimer’s Disease in a section of frozen Human normal brain.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND™. The section was incubated at room temperature for 1 hour with ab317594 at 1/100 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Tau Alzheimer's Disease antibody [GT-38] - Conformation-Specific (AB317594)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Tau Alzheimer's Disease antibody [GT-38] - Conformation-Specific (AB317594)

Immunofluorescence staining of Tau Alzheimer’s Disease in a section of frozen human Alzheimer brain.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND™. The section was incubated at room temperature for 1 hour with ab317594 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Carrier free

    Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific - BSA and Azide free

  • Unconjugated

    Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT-38

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB273617

Human Tau ELISA Kit

Recombinant|SimpleStep

ELISA - Human Tau ELISA Kit (AB273617)

  • 0
  • 0
Primary Antibodies

AB201679

Alexa Fluor® 647 Anti-CNPase antibody [11-5B]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CNPase antibody [11-5B] (AB201679)

  • 0
  • 0
Primary Antibodies

AB275189

Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224]

RabMAb|Recombinant|KO Validated

Flow Cytometry - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189)

  • 0
  • 0
Primary Antibodies

AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

  • 0
  • 0
Primary Antibodies

AB239459

Alexa Fluor® 647 Mouse IgG1 kappa [MOPC-21] - Isotype Control

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Mouse IgG1 kappa [MOPC-21] - Isotype Control (AB239459)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau protein also known as MAPT (Microtubule-Associated Protein Tau) plays a mechanical role in stabilizing microtubules in neuronal axons. The human tau protein has several isoforms generally weighing between 45 to 65 kDa. Tau is predominantly expressed in neurons of the central nervous system but can also be found in some non-neuronal cells. In neurons tau binds to tubulin and facilitates the polymerization and stability of microtubules which are essential for maintaining cellular structure and transport functions.
Biological function summary

Tau protein impacts the dynamics of the neuronal cytoskeleton. Tau is part of the intracellular transport system where it facilitates the movement of organelles and proteins along axons. This function is essential for neuron communication and resilience. Tau interacts with and regulates microtubule-associated complexes ensuring efficient intracellular transport. Its role in cytoskeletal integrity strongly influences cell signaling pathways critical for synaptic plasticity and neuronal survival.

Pathways

Tau protein plays a significant part in cellular transport and signal transduction pathways. One key pathway is the MAPK signaling pathway where tau's phosphorylation state influences neuronal response to external stimuli. Additionally tau is involved in the PI3K/AKT pathway affecting cell growth and survival. Within these pathways tau's phosphorylation is regulated by kinases including GSK-3β and CDK5 while phosphatases like PP2A dephosphorylate tau to maintain its functional state.

Dysfunctional tau is strongly linked to neurodegenerative diseases particularly Alzheimer's disease (AD) and frontotemporal dementia (FTD). In AD hyperphosphorylated tau forms neurofibrillary tangles which disrupt neuronal function and promote cell death. This process often involves other proteins such as beta-amyloid which exacerbate tau's pathological aggregation. Understanding tauopathy mechanisms has guided the research and development of anti-tau antibodies like anti-GT3 and anti-conformation antibodies as therapeutic strategies against tau-related pathologies in neurodegenerative disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com