AB214872

Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Thyroglobulin antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Thyroglobulin, Tg, TG

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (AB214872)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (AB214872)

ab214872 staining Thyroglobulin in TT cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.

The cells were then incubated overnight at +4°C with ab214872 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Unconjugated

Anti-Thyroglobulin antibody [EPR9730]
Carrier free

Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free
Carrier free

Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9730

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in TT cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroglobulin often abbreviated as Tg is a glycoprotein with a molecular mass of approximately 660 kDa. It is expressed primarily in the thyroid gland particularly within the follicular cells. This protein serves as a precursor for thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). High thyroglobulin levels in the bloodstream can indicate altered thyroid function and clinicians frequently measure these levels for diagnostic purposes. Researchers have developed specific anti-thyroglobulin antibodies to facilitate the study and measurement of this protein in various contexts.

Biological function summary

Thyroglobulin plays a critical role in the synthesis and storage of thyroid hormones. It acts as a scaffold for iodination and hormone synthesis within the colloid of thyroid follicles. Thyroglobulin is not part of a protein complex; rather it undergoes iodination to form hormone precursors that later convert into active hormones. Anti-thyroglobulin antibodies can be used in research or diagnostic labs to detect the presence or concentration of thyroglobulin in serum samples. These antibodies help in understanding the nuances of thyroid hormone biosynthesis and in monitoring thyroid activity.

Pathways

Thyroglobulin is deeply involved in the thyroid hormone synthesis pathway a fundamental part of the endocrine system's operations. This process connects closely with the iodine metabolism pathway as iodine plays an essential role in hormone production. Thyroglobulin interacts indirectly with proteins like thyroid peroxidase which catalyzes iodination of thyroglobulin. The synthesis and release of thyroid hormones proceed through a carefully orchestrated sequence affecting many physiological processes including metabolism growth and development.

Thyroglobulin has associations with conditions such as thyroid cancer and autoimmune thyroid diseases. For instance elevated serum thyroglobulin can serve as a tumor marker in differentiated thyroid cancer. Anti-thyroglobulin antibodies are often present in autoimmune thyroid diseases like Hashimoto's thyroiditis where the body's immune system mistakenly targets thyroid proteins. Thyroid peroxidase antibodies are also common in these disorders indicating a shared autoimmune response against thyroid-specific antigens. Understanding these relationships aids in the diagnosis and management of thyroid-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed : 17532758, PubMed : 32025030). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed : 32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed : 32025030). One dimer produces 7 thyroid hormone molecules (PubMed : 32025030).

See full target information Thyroglobulin

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of nuclear medicine and molecular imaging 52:4569-4581 PubMed40358697

2025

Development of a novel anti-CEACAM5 VHH for SPECT imaging and potential cancer therapy applications.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Cong,Rianne Biemans,Natasja G Lieuwes,Dennis Suijlen,Philippe Lambin,Ingrid Dijkgraaf,Matthias Bauwens,Ala Yaromina,Ludwig J Dubois

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (AB214872)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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