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AB309787

Alexa Fluor® 488 Anti-TLS/FUS antibody [EPR5812]

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Rabbit Recombinant Monoclonal TLS/FUS antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

TLS, FUS, RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TLS/FUS antibody [EPR5812] (AB309787)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TLS/FUS antibody [EPR5812] (AB309787)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling TLS/FUS with ab309787 at 1/50 (10.0 ug/ml) dilution (Green).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing nuclear staining in HepG2 cell line.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TLS/FUS antibody [EPR5812] (AB309787)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TLS/FUS antibody [EPR5812] (AB309787)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling TLS/FUS with ab309787 at 1/50 (10.0 ug/ml) dilution (Green).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing nuclear staining in K-562 cell line.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 578 PE

    PE Anti-TLS/FUS antibody [EPR5812]

  • 660 APC

    APC Anti-TLS/FUS antibody [EPR5812]

  • HRP

    HRP Anti-TLS/FUS antibody [EPR5812]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TLS/FUS antibody [EPR5812]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TLS/FUS antibody [EPR5812]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TLS/FUS antibody [EPR5812]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-TLS/FUS antibody [EPR5812]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-TLS/FUS antibody [EPR5812]

  • Unconjugated

    Anti-TLS/FUS antibody [EPR5812]

  • Carrier free

    Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5812

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLS/FUS also known as FUS and the FUS protein is an RNA-binding protein involved in various cellular processes. It has a molecular weight of approximately 53 kDa. Expressed extensively in the nucleus FUS/TLS relocates to the cytoplasm under stress conditions. It plays mechanical roles in transcription regulation RNA splicing and mRNA transport. Scientists sometimes refer to FUS as the 'fused in sarcoma' protein because of its involvement in gene fusion events.
Biological function summary

The FUS protein aids in the maintenance and stabilization of mRNA molecules and participates in the formation of stress granules distinct cytoplasmic aggregates of proteins and RNAs. It forms part of a large protein complex alongside other RNA-binding proteins. FUS stabilizes pre-mRNA structures which affects protein expression patterns essential for cell survival and differentiation. Its ability to bind to RNA and DNA indicates its fundamental role in genomic stability.

Pathways

The functions of FUS help regulate RNA metabolism-related pathways and cellular stress response pathways. FUS interacts with proteins like TAF15 and EWSR1 within these pathways showing a complex network of interactions that contribute to RNA maturation and stress granule dynamic formation. The activity of FUS in these pathways ultimately affects cellular homeostasis gene expression modulation and response to cellular stress.

FUS protein mutations or mislocalizations relate to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). These conditions are characterized by the accumulation of FUS or FUS-related aggregates in the cytoplasm leading to neuronal cell death. FUS is also connected to other RNA-binding proteins like TDP-43 which also mislocalizes and forms aggregates in these disorders highlighting the importance of protein homeostasis and normal RNA metabolism in the prevention of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response (PubMed : 27731383). Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing (PubMed : 26124092). Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay (PubMed : 24204307). Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (PubMed : 10567410). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity).
See full target information FUS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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