AB205486

Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(16 Publications)

Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 488. Mitochondrion marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 16 publications.

View Alternative Names

KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB205486)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB205486)

ab205486 staining TOMM20 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205486 at a 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free
Unconjugated

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
HRP

HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15581-39

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed : 40080546). Together with TOMM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (PubMed : 18331822). The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

See full target information TOMM20

style

left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29167 PubMed40783579

2025

Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of surgical digestive fistula.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Mariani,Augustin Guichard,Anna C Sebbagh,André Cronemberger Andrade,Zahra Al Amir Dache,Christopher Ribes,Dmitry Ayollo,Mehdi Karoui,Gregory Lavieu,Florence Gazeau,Amanda K A Silva,Gabriel Rahmi,Sabah Mozafari

Haematologica : PubMed40534505

2025

The STAT3-VDAC1 axis modulates mitochondrial function and plays a critical role in the survival of acute myeloid leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kellen B Gil,Jamie Borg,Rosana Moreira Pereira,Anagha Inguva-Sheth,Geovana Araujo,Jeremy Rahkola,William Showers,Abby Grier,Angelo D'Alessandro,Clayton Smith,Christine McMahon,Daniel A Pollyea,Austin E Gillen,Maria L Amaya

Cancer research 84:4049-4065 PubMed39288077

2024

mLumiOpto Is a Mitochondrial-Targeted Gene Therapy for Treating Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Patrick Ernst,Anusua Sarkar,Seulhee Kim,Yingnan Si,Tanvi Varadkar,Matthew D Ringel,Xiaoguang Margaret Liu,Lufang Zhou

Cell reports 42:113260 PubMed37851575

2023

A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S Waters,Sigurd B Angenent,Steven J Altschuler,Lani F Wu

Cancers 15: PubMed37345179

2023

Mass Spectrometry Imaging Reveals Abnormalities in Cardiolipin Composition and Distribution in Astrocytoma Tumor Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Anna C Krieger,Luis A Macias,J Clay Goodman,Jennifer S Brodbelt,Livia S Eberlin

The EMBO journal 42:e111699 PubMed36912136

2023

Inhibition of ATP synthase reverse activity restores energy homeostasis in mitochondrial pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Acin-Perez,Cristiane Benincá,Lucia Fernandez Del Rio,Cynthia Shu,Siyouneh Baghdasarian,Vanessa Zanette,Christoph Gerle,Chimari Jiko,Ramzi Khairallah,Shaharyar Khan,David Rincon Fernandez Pacheco,Byourak Shabane,Karel Erion,Ruchi Masand,Sundeep Dugar,Cristina Ghenoiu,George Schreiner,Linsey Stiles,Marc Liesa,Orian S Shirihai

EMBO reports 23:e54746 PubMed36156348

2022

MCU controls melanoma progression through a redox-controlled phenotype switch.

Applications

Unspecified application

Species

Unspecified reactive species

Ioana Stejerean-Todoran,Katharina Zimmermann,Christine S Gibhardt,Adina Vultur,Christian Ickes,Batool Shannan,Zuriñe Bonilla Del Rio,Anna Wölling,Sabrina Cappello,Hsu-Min Sung,Magdalena Shumanska,Xin Zhang,Maithily Nanadikar,Muhammad U Latif,Anna Wittek,Felix Lange,Andrea Waters,Patricia Brafford,Jörg Wilting,Henning Urlaub,Dörthe M Katschinski,Peter Rehling,Christof Lenz,Stefan Jakobs,Volker Ellenrieder,Alexander Roesch,Michael P Schön,Meenhard Herlyn,Hedwig Stanisz,Ivan Bogeski

Frontiers in cardiovascular medicine 9:778919 PubMed35155626

2022

Lipoprotein(a) Induces Vesicular Cardiovascular Calcification Revealed With Single-Extracellular Vesicle Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Maximillian A Rogers,Samantha K Atkins,Kang H Zheng,Sasha A Singh,Sarvesh Chelvanambi,Tan H Pham,Shiori Kuraoka,Erik S G Stroes,Masanori Aikawa,Elena Aikawa

Cell death & disease 12:271 PubMed33723235

2021

NIK promotes metabolic adaptation of glioblastoma cells to bioenergetic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L Kamradt,Ji-Ung Jung,Kathryn M Pflug,Dong W Lee,Victor Fanniel,Raquel Sitcheran

Nature communications 11:5339 PubMed33087718

2020

Dual mRNA therapy restores metabolic function in long-term studies in mice with propionic acidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Jiang,Ji-Sun Park,Ling Yin,Rodrigo Laureano,Eric Jacquinet,Jinsong Yang,Shi Liang,Andrea Frassetto,Jenny Zhuo,Xinhua Yan,Xuling Zhu,Steven Fortucci,Kara Hoar,Cosmin Mihai,Christopher Tunkey,Vlad Presnyak,Kerry E Benenato,Christine M Lukacs,Paolo G V Martini,Lin T Guey

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB205486)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

Product promise