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Rabbit Recombinant Monoclonal Transaldolase 1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (AB322240), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (AB322240), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (AB322240), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (AB322240), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (AB322240), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate (PubMed:18687684, PubMed:8955144). Not only acts as a pentose phosphate pathway enzyme, but also affects other metabolite pathways by altering its subcellular localization between the nucleus and the cytoplasm (By similarity).

Alternative names

TAL, TALDO, TALDOR, TALDO1, Transaldolase

Recommended products

Rabbit Recombinant Monoclonal Transaldolase 1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28140-51
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Transaldolase 1 also known as TALDO1 or TAL-H is an enzyme that plays an important role in the pentose phosphate pathway by catalyzing the transfer of a three-carbon dihydroxyacetone unit from sedoheptulose 7-phosphate to glyceraldehyde 3-phosphate producing erythrose 4-phosphate and fructose 6-phosphate. The molecular mass of Transaldolase 1 is approximately 37 kDa. It is expressed in various tissues within the body with higher expression levels observed in the liver and adipose tissues.

Biological function summary

Transaldolase 1 regulates the balance between the oxidative and non-oxidative branches of the pentose phosphate pathway essential for nucleotide biosynthesis and maintaining cellular redox status. It functions independently not as part of a larger enzyme complex. The activity of this enzyme affects cellular metabolic functions impacting the synthesis of nucleotides and pentose sugars which are necessary for cell growth and division.

Pathways

Transaldolase 1 is integral to the pentose phosphate pathway also known as the hexose monophosphate shunt. This pathway is important for providing ribose-5-phosphate for nucleotide synthesis and NADPH for reductive biosynthesis and cellular antioxidant systems. It links with glycolysis through shared intermediates such as fructose 6-phosphate connecting with enzymes like glucose-6-phosphate dehydrogenase. This relationship allows cells to adjust their metabolic flux according to physiological needs.

Associated diseases and disorders

Transaldolase 1 mutations are associated with transaldolase deficiency a rare metabolic disorder that can lead to liver dysfunction and immunodeficiency. This deficiency affects the cellular ability to balance nucleotide and lipid biosynthesis contributing to the disease phenotype. There is also a connection with oxidative stress-related disorders as Transaldolase 1 indirectly influences reactive oxygen species via its role in NADPH production which ties it to proteins involved in oxidative stress responses like NADPH oxidase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab322240 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab322240 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transaldolase 1 with ab322240 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on human liver.

    The section was incubated with ab322240 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Transaldolase 1 with ab322240 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on human liver cancer.

    The section was incubated with ab322240 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Transaldolase 1 with ab322240 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on mouse liver.

    The section was incubated with ab322240 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Transaldolase 1 with ab322240 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on rat liver.

    The section was incubated with ab322240 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab322240 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing nuclear staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transaldolase 1 antibody [EPR28140-51] (ab322240)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab322240 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing nuclear staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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