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AB198595

Alexa Fluor® 488 Anti-TRF2 antibody [EPR3517(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal TRF2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

TRBF2, TRF2, TERF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TRF2 antibody [EPR3517(2)] (AB198595)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TRF2 antibody [EPR3517(2)] (AB198595)

ab198595 staining TRF2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198595 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TRF2 antibody [EPR3517(2)] (AB198595)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TRF2 antibody [EPR3517(2)] (AB198595)

Overlay histogram showing HeLa cells stained with ab198595 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198595, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3517(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRF2 also known as Telomeric Repeat-binding Factor 2 is a protein important for telomere protection and maintenance. TRF2 weighs approximately 55 kDa and is widely expressed in human tissues especially in those with high turnover rates. This protein binds specifically to double-stranded telomeric DNA and forms a protective cap at the end of chromosomes helping to regulate telomere length and integrity. By securing the telomere's structure TRF2 prevents chromosomes from being recognized as damaged DNA.
Biological function summary

TRF2 plays a central role in telomere function by assembling into the shelterin complex. This complex which includes other proteins like TRF1 TIN2 POT1 TPP1 and RAP1 is essential for controlling telomere length and stability. TRF2 is responsible for inhibiting DNA damage response pathways at telomeres ensuring that the natural chromosomal ends are not mistaken for double-strand breaks. This suppression of unwanted DNA damage response is important for normal cell division and avoiding cellular senescence.

Pathways

Researchers have placed TRF2 within the DNA repair and telomere maintenance pathways. TRF2 interacts with several other proteins such as Ku70/80 and RAD51 that are instrumental in the DNA repair process. Furthermore TRF2 influences the Alternative Lengthening of Telomeres (ALT) pathway which is a telomere lengthening process found in some cancer cells. These connections highlight TRF2's role in maintaining genomic stability through its capacity to regulate telomere-associated proteins and pathways.

TRF2 has been linked to cancer and aging. Elevated levels of TRF2 have been detected in various cancers including breast and prostate cancer suggesting a role in tumorigenesis where it contributes to the stability of cancer cell telomeres. Additionally defects in TRF2 or its interaction with partner proteins like POT1 can lead to dysfunctional telomeres which contribute to the aging process and age-related diseases by affecting cell proliferation and survival. These links demonstrate the importance of TRF2 in normal cellular function and its potential as a therapeutic target in disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes (PubMed : 15608617, PubMed : 16166375, PubMed : 20655466, PubMed : 28216226, PubMed : 9326950, PubMed : 9326951, PubMed : 9476899). In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo (PubMed : 16166375, PubMed : 20655466). Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection (PubMed : 16166375). Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways (PubMed : 16166375). Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres : T loops have been proposed to protect chromosome ends from degradation and repair (PubMed : 20655466). Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo (PubMed : 20655466, PubMed : 28216226). Preferentially binds to positive supercoiled DNA (PubMed : 15608617, PubMed : 20655466). Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology (PubMed : 20655466). Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length (By similarity).
See full target information TERF2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:82 PubMed36737598

2023

Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingli Liu,Jingyuan Xu,Linyao Lu,Lili Gao,Shengjuan Zhu,Yi Sui,Ting Cao,Tao Yang

ACS pharmacology & translational science 3:1253-1264 PubMed33344901

2020

Selective Elimination of Osteosarcoma Cell Lines with Short Telomeres by Ataxia Telangiectasia and Rad3-Related Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Goncalves,Georgia Zoumpoulidou,Carlos Alvarez-Mendoza,Caterina Mancusi,Laura C Collopy,Sandra J Strauss,Sibylle Mittnacht,Kazunori Tomita
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Product promise

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