AB206726

Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal TTF1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB206726)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB206726)

IHC image of TTF1/Nkx2-1 staining in a section of formalin-fixed paraffin-embedded normal human thyroid*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab206726 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]
Unconjugated

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]
Carrier free

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5955(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.

Biological function summary

TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.

Pathways

The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.

TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

See full target information NKX2-1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 41:871-886.e10 PubMed37059105

2023

Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgio Gaglia,Megan L Burger,Cecily C Ritch,Danae Rammos,Yang Dai,Grace E Crossland,Sara Z Tavana,Simon Warchol,Alex M Jaeger,Santiago Naranjo,Shannon Coy,Ajit J Nirmal,Robert Krueger,Jia-Ren Lin,Hanspeter Pfister,Peter K Sorger,Tyler Jacks,Sandro Santagata

Cell 184:4996-5014.e26 PubMed34534464

2021

Antigen dominance hierarchies shape TCF1 progenitor CD8 T cell phenotypes in tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Megan L Burger,Amanda M Cruz,Grace E Crossland,Giorgio Gaglia,Cecily C Ritch,Sarah E Blatt,Arjun Bhutkar,David Canner,Tamina Kienka,Sara Z Tavana,Alexia L Barandiaran,Andrea Garmilla,Jason M Schenkel,Michelle Hillman,Izumi de Los Rios Kobara,Amy Li,Alex M Jaeger,William L Hwang,Peter M K Westcott,Michael P Manos,Marta M Holovatska,F Stephen Hodi,Aviv Regev,Sandro Santagata,Tyler Jacks

eLife 8: PubMed31309927

2019

Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Kh Stavoe,Pallavi P Gopal,Andrea Gubas,Sharon A Tooze,Erika Lf Holzbaur

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB206726)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise