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Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TUBA4A antibody [EPR13477(B)] - Microtubule Marker (AB307611), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TUBA4A antibody [EPR13477(B)] - Microtubule Marker (AB307611), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PICC/IF
Human
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13477(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TUBA4A also known as Tubulin Alpha 4A helps in the polymerization of microtubules acting as a core component of the cytoskeleton. It weighs approximately 50 kDa. This protein is expressed in various tissues including the nervous system and muscles. It plays a role in maintaining cellular structure and functions in cellular transport and division.

Biological function summary

Tubulin Alpha 4A is involved in the dynamic assembly and disassembly of microtubules contributing to cell shape and intracellular transport. It forms part of the microtubule complex and interacts with other tubulin isoforms to provide stability and flexibility to microtubule structures. This protein is essential for dividing cells as it supports the mitotic spindle's function during cell division.

Pathways

The TUBA4A protein actively participates in cytoskeleton regulation and signal transduction. It is part of the MAPK signaling pathway important for cell division and differentiation. Components like MAP kinases and related tubulins work together within these pathways to mediate cellular responses to various stimuli facilitating critical cell functions and adaptability.

Associated diseases and disorders

TUBA4A has associations with neurodegenerative conditions and cancers. Mutations in TUBA4A have a link to amyotrophic lateral sclerosis (ALS) where disruptions in microtubule function lead to neuronal damage. Additionally aberrant expression of TUBA4A may contribute to tumor progression where interactions with proteins like beta-tubulin disrupt cellular adhesion and division mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TUBA4A antibody [EPR13477(B)] - Microtubule Marker (ab307611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TUBA4A antibody [EPR13477(B)] - Microtubule Marker (ab307611)

    TUBA4A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human breast using rabbit Anti-TUBA4A antibody

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TUBA4A with ab307611 at 1/50 (10.0 μg/ml). Positive staining on human breast. The section was incubated with ab307611 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TUBA4A antibody [EPR13477(B)] - Microtubule Marker (ab307611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-TUBA4A antibody [EPR13477(B)] - Microtubule Marker (ab307611)

    TUBA4A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human endometrium using rabbit Anti-TUBA4A antibody

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling TUBA4A with ab307611 at 1/50 (10.0 μg/ml). Positive staining on human endometrium. The section was incubated with ab307611 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com