Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195883)

ab195883 staining tubulin in HeLa cells. The cells were fixed with fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195883 at a 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195883)

ab303579 staining FOXC1 in wild-type HEK293 (Human epithelial cell line from embryonic kidney) cells (top panel) and FOXC1 knockout HEK293 cells (bottom panel). The cells were fixed with paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab303579 at 1 µg/ml (shown in red) and ab195883 (anti-tubulin, shown in green) at 1/100 dilution overnight at +4°C. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195883)

ab303598 staining CD45 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (positive control) and HEK293 cells (negative control). The cells were fixed with 100% methanol (10 min) permeabilized with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab303598  at 5 µg/ml (shown in red) and ab195883 (Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34]) at 5 µg/ml (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195883)

ab199200 staining CD151 in HT1080 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab199200 at 2µg/ml (shown in magenta) and ab195883, Rat monoclonal [YOL1/34] to alpha Tubulin 1 - Microtubule Marker (Alexa Fluor^® 488), pre-adsorbed at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.