JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB206097

Alexa Fluor® 488 Anti-UAP56 antibody [EPR13144-62]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal UAP56 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BAT1, UAP56, DDX39B, Spliceosome RNA helicase DDX39B, 56 kDa U2AF65-associated protein, ATP-dependent RNA helicase p47, DEAD box protein UAP56, HLA-B-associated transcript 1 protein

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-UAP56 antibody [EPR13144-62] (AB206097)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-UAP56 antibody [EPR13144-62] (AB206097)

ab206097 staining UAP56 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206097 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13144-62

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UAP56 also known as DDX39B or SUB2 is an ATP-dependent RNA helicase with a molecular mass of approximately 46 kDa. It plays a significant role in RNA processing by unwinding RNA duplexes. UAP56 is found throughout the nucleus and functions in various cell types with high expression levels in actively dividing cells. It binds RNA substrates using ATP hydrolysis facilitating movement along RNA strands and this action is vital for splicing and mRNA export from the nucleus to the cytoplasm.
Biological function summary

This protein contributes to the assembly of the spliceosome complex which is essential for pre-mRNA splicing. By interacting with other splicing factors UAP56 ensures accurate removal of introns and the joining of exons in the mRNA. Its function is also necessary for the proper export of mRNA through the nuclear pore complex. Through these activities UAP56 supports gene expression by ensuring that only mature mRNA is available for translation.

Pathways

UAP56 operates in the mRNA processing and transport pathways. In the mRNA export pathway it collaborates with other proteins like Aly/REF and the TREX complex facilitating the translocation of mRNA from the nucleus to the cytoplasm. In the splicing pathway UAP56 interacts with small nuclear ribonucleoproteins (snRNPs) to ensure correct splicing. These interactions help maintain the efficiency and fidelity of gene expression.

Defects in UAP56 or its interactions may contribute to cancer and neurodegenerative diseases. In cancer abnormal mRNA export and splicing can lead to the expression of oncogenes or the loss of tumor suppressors implicating UAP56 in tumor progression. In neurodegenerative disorders UAP56 dysfunction might result in improper mRNA processing leading to the accumulation of misfolded proteins. Its role is related to proteins such as TDP-43 which is involved in neurodegenerative pathologies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in nuclear export of spliced and unspliced mRNA (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). The TREX complex is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). The THOC1-THOC2-THOC3 core complex alone is sufficient to promote ATPase activity of DDX39B; in the complex THOC2 is the only component that directly interacts with DDX39B (PubMed : 33191911). Associates with SARNP/CIP29, which facilitates RNA binding of DDX39B and likely plays a role in mRNA export (PubMed : 37578863). May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC4 and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability.. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed : 23299939] reporting a stimulatory effect.. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
See full target information DDX39B
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309220 PubMed39023197

2024

A Linkable, Polycarbonate Gut Microbiome-Distal Tumor Chip Platform for Interrogating Cancer Promoting Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle S K Brasino,Sean D Speese,Kevin Schilling,Carolyn E Schutt,Michelle C Barton
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB181059

Anti-UAP56 antibody [EPR13144]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP56 antibody [EPR13144] (AB181059)

  • 0
  • 0
Primary Antibodies

AB181061

Anti-UAP56 antibody [EPR13144-62]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-UAP56 antibody [EPR13144-62] (AB181061)

  • 0
  • 0

Complementary Products

Proteins & Peptides

AB99297

Recombinant Human UAP56 protein

SDS-PAGE - Recombinant Human UAP56 protein (AB99297)

  • 0
  • 0
Assay Kits

AB267631

Human HGFA ELISA Kit

Sandwich ELISA - Human HGFA ELISA Kit (AB267631)

  • 0
  • 0
Proteins & Peptides

AB139631

Recombinant Human LCAT protein (denatured)

SDS-PAGE - Recombinant Human LCAT protein (denatured) (AB139631)

  • 0
  • 0
Biochemicals

AB145200

R-(+)-DIOA (R(+)-Butylindazone), K+/Cl--cotransporter inhibitor

Chemical Structure - R-(+)-DIOA (R(+)-Butylindazone), K+/Cl--cotransporter inhibitor (AB145200)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com