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AB202828

Alexa Fluor® 488 Anti-VAMP8/EDB antibody [EP2629Y]

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(2 Publications)

Rabbit Recombinant Monoclonal VAMP8/EDB antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-VAMP8/EDB antibody [EP2629Y] (AB202828)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-VAMP8/EDB antibody [EP2629Y] (AB202828)

ab202828 staining VAMP8/EDB in Jurkat cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 578 PE

    PE Anti-VAMP8/EDB antibody [EP2629Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2629Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Jurkat cells fixed with 100% methanol (5 min).</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAMP8 also known as synaptobrevin-like protein 2 or EDB is a SNARE protein with a mass of approximately 12 kDa. This protein is widely expressed in many tissues including the lung pancreas and kidney. It plays an important role in vesicle-mediated transport where it facilitates the fusion of vesicles with target membranes as part of a larger protein complex. By participating in these membrane fusion processes the protein contributes to cellular trafficking.
Biological function summary

VAMP8 engages in vesicular transport mechanisms that support exocytosis and endocytosis processes. It forms part of the SNARE complex which is important for merging vesicles with their target compartments. Through this association VAMP8 aids the release of substances such as hormones and digestive enzymes from the cells. This process is critical for maintaining the balance and function of cellular activities across different tissues.

Pathways

VAMP8 functions in the context of the trafficking pathways that include the endocytic and exocytic pathways. It is a participant in regulated exocytosis where it interacts with other SNARE proteins like syntaxin and SNAP-25 ensuring precise membrane fusion events occur. These pathways ensure proper cellular communication and debris clearance underlining the importance of VAMP8 in general cellular homeostasis.

VAMP8 is associated with conditions like inflammatory diseases and certain exocytic disorders. Researchers have observed its involvement in conditions like pancreatitis where its regulatory function in enzyme secretion becomes apparent. VAMP8 also has connections with other disease-related proteins such as those in the SNARE family which contribute to altered secretory pathways and inflammation. Understanding the precise mechanisms of VAMP8 helps in developing therapeutic approaches to managing these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed : 23217709, PubMed : 25686604). Also required for dense-granule secretion in platelets (PubMed : 12130530). Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity). Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (PubMed : 31694946).
See full target information Vesicle-associated membrane protein 8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8519 PubMed39353976

2024

CHIP ameliorates nonalcoholic fatty liver disease via promoting K63- and K27-linked STX17 ubiquitination to facilitate autophagosome-lysosome fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunjin Rho,Seungyeon Kim,Seung Up Kim,Jeong Won Kim,Sang Hoon Lee,Sang Hoon Park,Freddy E Escorcia,Joon-Yong Chung,Jaewhan Song

Advanced materials (Deerfield Beach, Fla.) 31:e1900401 PubMed30920710

2019

Wet/Sono-Chemical Synthesis of Enzymatic Two-Dimensional MnO Nanosheets for Synergistic Catalysis-Enhanced Phototheranostics.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Tang,Wenpei Fan,Weizhong Zhang,Zhen Yang,Ling Li,Zhantong Wang,Ya-Ling Chiang,Yijing Liu,Liming Deng,Liangcan He,Zheyu Shen,Orit Jacobson,Maria A Aronova,Albert Jin,Jin Xie,Xiaoyuan Chen
View all publications
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