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AB309860

Alexa Fluor® 488 Anti-VAV2 antibody [EP1067Y]

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Rabbit Recombinant Monoclonal VAV2 phospho Y172 antibody - conjugated to Alexa Fluor® 488.

View Alternative Names

Guanine nucleotide exchange factor VAV2, VAV-2, VAV2

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1067Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAV2 also known as Vav 2 guanine nucleotide exchange factor is a member of the VAV family of proteins which function as guanine nucleotide exchange factors (GEFs). It has a molecular mass of approximately 100 kDa. The protein converts inactive GDP-bound Rho family GTPases such as Rac1 and Cdc42 into their active GTP-bound form. VAV2 is expressed in various tissues including the brain heart and lung indicating its broad role in cellular signaling across different systems.
Biological function summary

VAV2 influences multiple cellular processes through its role as a GEF. This protein does not form a part of a static complex but interacts dynamically with other signaling components. VAV2 regulates cytoskeleton organization cell migration and growth by activating Rho family GTPases. Its activity impacts processes such as neuronal development and immune response by modifying the actin cytoskeleton which is important for cell movement and morphological changes.

Pathways

VAV2 plays an integral role in signaling cascades like the Ras and MAPK pathways. It interacts closely with proteins like Rac1 and Cdc42 to propagate signals that influence cell proliferation and survival. By facilitating the switch from inactive GDP-bound states to active GTP-bound forms of Rho GTPases VAV2 integrates signals from cell surface receptors to downstream effectors that mediate cellular responses to external stimuli.

VAV2’s function connects to cancer progression and cardiovascular disorders. Changes in VAV2 expression or activity can lead to abnormal cell growth and differentiation linking it to cancerous transformations. Additionally because VAV2 modulates cell migration and cytoskeletal dynamics its malfunction can contribute to cardiovascular diseases by affecting vascular smooth muscle cells and endothelial cells. In both contexts VAV2's interactions with Rho family proteins like Rac1 highlights its influence in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).
See full target information Guanine nucleotide exchange factor VAV2 phospho Y172
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Product promise

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For full details, please see our Terms & Conditions

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