AB225443

Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal VE Cadherin antibody - conjugated to Alexa Fluor® 488. Intercellular Junction marker. Suitable for ICC/IF and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

CD144, Cadherin-5, Vascular endothelial cadherin, VE-cadherin, Cdh5

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB225443)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB225443)

ab225443 staining VE Cadherin in bEnd.3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225443 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker
Unconjugated

Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker
Carrier free

Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (Capture)
Carrier free

Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18229

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse, Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in bEnd.3 cells fixed with 4% formaldehyde (10 min)</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity). This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (PubMed : 20332120, PubMed : 9220534). It associates with alpha-catenin forming a link to the cytoskeleton (By similarity). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (PubMed : 24806444). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (PubMed : 27466317). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (By similarity). Positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechantransduction (By similarity). Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (By similarity). Associates with CTNND1/p120-catenin to control CADH5 endocytosis (PubMed : 33972531).

See full target information Cdh5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of ginseng research 49:260-270 PubMed40453348

2025

Ginsenoside Rg5 alleviates radiation-induced acute lung vascular endothelium injury by reducing mitochondrial apoptosis via Sirt1.

Applications

Unspecified application

Species

Unspecified reactive species

Churong Li,Biao Zhao,Jing Xiong,Linjie Li,Dalong Pang,Keith Unger,Mira Jung,Jiahua Lyu,Hao Kuang,Long Liang,Tao Li,Long Chen,Hansong Bai

Heliyon 10:e27494 PubMed38515687

2024

Regulation of Dihydroartemisinin on the pathological progression of laryngeal carcinoma through the periostin/YAP/IL-6 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Zhang,Rui-Cong Li,Cong Xu,Xiao-Ming Li

Nature communications 12:7079 PubMed34873173

2021

A tough nitric oxide-eluting hydrogel coating suppresses neointimal hyperplasia on vascular stent.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Chen,Peng Gao,Lu Huang,Xing Tan,Ningling Zhou,Tong Yang,Hua Qiu,Xin Dai,Sean Michael,Qiufen Tu,Nan Huang,Zhihong Guo,Jianhua Zhou,Zhilu Yang,Hongkai Wu

Frontiers in molecular biosciences 8:682367 PubMed34179086

2021

The Inhibitory Effect of Lysophosphatidylcholine on Proangiogenesis of Human CD34 Cells Derived Endothelial Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haijun Zhao,Yanhui He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB225443)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise